2). e Values of σβ, corresponding to the relative rate of conduction at the inner pore close to the Ca2+ binding site (see “Methods”), for mutants displaying basal activity at zero and saturating Ca2+ concentrations. Dashed line indicates the value of WT at saturating Ca2+ ...
The anion channel TMEM16A is activated by intracellular Ca2+ in a highly cooperative process. By combining electrophysiology and autocorrelation analysis, we investigated the mechanism of channel activation and the concurrent rearrangement of the gate in