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43 The microscope was equipped with a multi-valve perfusion system (VC3-8xG, ALA Scientifics instruments) and an inline heater (SF-28, Warner) so that neurons were continuously perfused with heated (32 °C) solutions. Transfected neurons were imaged with a Toptica iChrome MLE laser at 488 ...
Both liquid A and the citric acid solution were lead by peristaltic pumps to a small mixing chamber (volume 26 ml) containing a high energy mixer device (Ultraturrax UTL 25 basic inline) set at 24,000 RPM. The flow of the citric acid solution was 167 g/minute. The flow of liquid A ...
Both liquid A and the citric acid solution were lead by peristaltic pumps to a small mixing chamber (volume 26 ml) containing a high energy mixer device (Ultraturrax UTL 25 basic inline) set at 24,000 RPM. The flow of the citric acid solution was 167 g/minute. The flow of liquid A ...
(M-VP-25XA-XYZL, Newport) carried the mice for a proper ROI. For two-photon validation experiments, the correction ring of the 25× water immersion objective was adjusted to compensate for the coverslip thickness and eliminate spherical aberrations. The highest excitation power of two-photon ...
using both inline and bath heaters. Whole-cell electrophysiology Electrophysiological data were acquired using a Multiclamp 700B amplifier (Molecular Devices, Sunnyvale, CA, USA), filtered at 2 kHz and digitized at 10/20 kHz using a Digidata 1440 digitizer (Molecular Devices) controlled by ...
Two ultrasensitive GaAsP PMTs (Hamamatsu, Bridgewater, NJ, USA) with bandpass 525/50 nm and 605/70 nm filter cubes (Chroma Technology, Bellows Falls, VT, USA) were used for collecting red/green signal. An Olympus XLUMPlanFLN 20×/1.0 water objective (WD = 2 mm) was used for image ...