Degenerative aortic stenosis (AS) is the most frequent valvular heart disease in Western societies and exhibits an increasing incidence due to ageing of the population [1]. Transcatheter aortic valve implantation (TAVI) improves survival of patients with severe AS with or without left ventricular (LV...
He underwent a dual chamber pacemaker for sick sinus syndrome at 54 years of age. Now he presented with progressive functional class IV dyspnoea. Chest X-ray revealed a suspicious area of patchy calcification in the cardiac silhouette ( Figure 1A ). Echocardiography showed moderate systolic left ...
Both types of diabetes can lead to serious complications if not properly managed, including cardiovascular disease, kidney disease (nephropathy), nerve damage (neuropathy), and eye damage (retinopathy) [3,4]. Diabetes significantly increases the risk of cardiovascular events such as heart attack, str...
Both types of diabetes can lead to serious complications if not properly managed, including cardiovascular disease, kidney disease (nephropathy), nerve damage (neuropathy), and eye damage (retinopathy) [3,4]. Diabetes significantly increases the risk of cardiovascular events such as heart attack, str...
were seeded into the lower chamber of the 6-well plate. Both AFs and VSMCs were cultured with the exosome-depleted medium containing 0.9 mM inorganic phosphorus or 3.5 mM inorganic phosphorus. After 72 h, the total cellular proteins of VSMCs in the lower chamber were collected for detection...
CUS and CAS fibroblasts (1.5 × 103cells/well) were seeded on 20 µg/ml collagen-I (A10483, Gibco) coated glass chamber slides. After 24 hours cells were fixed in 4% (v/v) paraformaldehyde in DPBS, permeabilized with 0.5% Tween-20 in DPBS for 20 min at room temperature...
After trypsinization, the cells were counted manually in a counting chamber. The following formula was used to calculate PDT: PDT = (T2-T1)*log (2)/Log (P2/P1) (Td = doubling time, P1 = the number of cell at T1, P2 = the number of cell at T2). 2.4. miRNA...
The Transwell chambers (pore size of 8 μM; Coring Inc., NY, USA) were placed on a 24-well plate, 0.2 mL of cell suspension was added to the upper chamber, and 600 μL complete medium was added to the lower chamber. After the cell culture was continued for 24 h, non-...
Cardiac computed tomography; Cardiac magnetic resonance Abstract Mitral annulus calcification is a common echocardiographic finding, particularly in the elderly and in end-stage renal disease patients under chronic dialysis. Caseous calcification or liquefaction necrosis of mitral annulus calcificati...
A dynamic flow type testing to study calcification was self-designed to investigate calcification in bioprosthetic heart valves. The apparatus consists of a container into which leaflets from a porcine aortic valve are placed, a chamber that contains calcium solution, and a peristaltic pump that ...