CAG-Cas9: R26-CAG-Cas9 mice (NM-KI-00120) obtained by R26-CAG-LNL-Cas9(NM-KI-00038) crossed with Ella-Cre Cas9-krab: R26-CAG-3XKRAB-dCas9-IRES-EGFP(NM-KI-18007) Fig2. Detection of CAS9 expression in R26-CAG-CAS9
pCAG Cas9-2A-Citrine (Plasmid #92358) HH-CAS-160pU6-pegRNA-GG-acceptor(Plasmid #132777) HH-CAS-159pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)(Plasmid #140003) HH-CAS-157PB-TRE-dCas9-KRAB-MeCP2(Plasmid #122267) HH-CAS-158Lenti-eCas9(Plasmid #140237) HH-CAS-158 HH-CAS-...
利用CRISPR基因编辑技术,将CAG-LSL-Cas9-FRT-IRES-tdTomato-FRT-WPRE-polyA插入到大鼠Rosa26基因中。 应用领域:Cas9大鼠,基因编辑 *使用本品系发表的文献需注明: R26-CAG-LSL-Cas9-IRES-tdTomato(SD) rats (Cat. NO. NR-KI-200004) were purchased from Shanghai Model Organisms Center, Inc.. ...
求助：请问哪位大神有129-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh/J这个小鼠？实验急用，谢谢。
亨廷顿舞蹈病(Huntington’s Disease, HD)是一种由HTT基因中CAG重复序列扩展引起的常染色体显性遗传神经退行性疾病。尽管CAG重复长度是决定发病年龄的主要因素,但体细胞中的CAG扩展加剧了疾病的进展。近日,Ric…
为了筛选影响CAG重复扩增的遗传修饰因子,该团队构建了一个基于CRISPR-Cas9的体内基因编辑平台,利用腺相关病毒(AAV)递送sgRNA,在组成型表达Cas9的亨廷顿病小鼠模型中进行基因编辑,高效敲除目标基因并观察其对CAG重复扩增的影响。该团队首先针对...
CAG repeat in genetically engineered pigs mimicking the selective neurodegeneration seen in patients with HD. A single intracranial or intravenous injection of adeno-associated virus encoding for Cas9, a single-guide RNA targeting theHTTgene, and donor DNA containing the normal CAG repeat led to the...
We have previously demonstrated that the Cas9 D10A nickase can effectively contract CAG/CTG repeats when targeted to the repeat tract itself. However, the mechanism remains unclear. Here, we tested whether nickase-mediated contractions depend on transcription or on replication using human cell models...
pCAG Cas9-2A-Citrine质粒 货号:#92358-20ul 货号产品名称规格库存价格数量购买 #92358-20ulpCAG Cas9-2A-Citrine质粒20ul 立即咨询 +- 产品介绍 相关产品 》产品信息 货号名称规格 #92358pCAG Cas9-2A-Citrine质粒 20ul 》产品描述 基因编辑 》产品简介 ...
产品名称: pCAGCas9-2A-Citrine哺乳编辑Cas9表达质粒 产品编号: P1804 规格: 10μl质粒/300μl菌液 原核抗性: 氨苄青霉素Amp 克隆菌株: 大肠杆菌DH5α 培养条件: 37℃ 质粒简介: pCAG Cas9-2A-Citrine是一个哺乳细胞基因编辑Cas9表达质粒。 质粒图谱: 质粒序列: 点击查看序列 ▍购买记录(近期成...