CAA motif interruptions decrease the stability of CAG hairpin as indicated by higher ΔG in predicted secondary structure (Additional file 1: Figure S2). These differences can influence the set of RNA-binding pro- teins interacting with ATXN2 RNAs, and possibly inter- fere with RNA processing,...
1976年,Piepolo和Zuffardi通过细胞遗传学研究发现无精症患者有Y染色体长臂的缺失,因此推测Yq11存在“染色体精子生成基因”,即AZF因子。后来在Yq11的5～6间隔中,发现有许多位点与精子发生相关,分别命名为AZFa、AZFb和AZFc,它们的侯选者分别为DFFRY、RMB(RNA-binding motif, RNA识别基序)和DAZ(deleted in azoospermia)...
CagI was even no longer detectable at all in whole cell lysates of the ΔL DQNT motif mutant (Fig. 2), in contrast to the cagL knock-out mutant, which still had detectable levels of CagI. Three deletion mutants, ΔR GD, ΔA EQQC, and ΔTASLI, displayed ...
et al. A Helical RGD Motif Promoting Cell Adhesion: Crystal Structures of the Helicobacter pylori Type IV Secretion System Pilus Protein CagL. Structure. 21, 1931–1941 (2013). CAS PubMed Google Scholar Barden, S. et al. Structure of a three-dimensional domain-swapped dimer of the ...
The three dimensional structures of the C_1X_2G_3(X_3)X_4C_5 motif of hCG, which is considered to be important for noncovalent assembly of the α- and β-subunits of glycoprotein hormones were analyzed to assess the importance of glycine (Gly) (G) at site X_3 in the motif by the...
Point mutations of the gene coding for the voltage-dependent calcium channel subunit are responsible for familial hemiplegic migraine (FHM) and episodic ataxia type 2 (EA2). In addition, expansions of the CAG repeat motif at the 3′ end of the gene, smaller than those responsible for dynamic...
[19,20] than in wild type cells. Similarly, both CSB-null and helicase motif V/VI mutant cells showed a lower activity for 8-oxoG incision in nuclear DNA than cells carrying a functional CSB [21,22]. Lower incision level for 8-oxoG in CS cells correlated with reduced expression level ...
Like many TLS polymerases, pol β possesses a PCNA-interacting protein (PIP) motif and can interact with PCNA (33), an essential property for polymerases to participate in polymerase switching (19). As a key enzyme involved in DNA base excision repair, pol β has been implicated in CAG ...
Nuclear Magnetic Resonance (NMR) experiments for RNA duplex with a single CAG motif, 5′-rCrCrGrCrArGrCrGrG-3′ were performed on a high-resolution AVANCE III 400 or 700 MHz BioSpin International AG, Switzerland as reported previously23. RNA samples were prepared in an appropriate buffer, ...
It has been previously shown that structural RNA elements in which the initiation AUG codon is embedded have a strong impact on translation i.e., placing an AUG codon within a highly stable hairpin motif substantially reduced translational efficiency.86, 87, 88, 89, 90 Nevertheless, despite the...