Modified single-stranded oligonucleotides were used to direct the nucleotide exchange of an A residue to a T residue in the second codon of the HD-gene, resulting in the creation of a leucine residue among the poly Q tract. Consistent with results from other groups, we provide evidence that ...
Near the N-terminus of the mature human DNA polymerase is a stretch of 13 glutamine residues encoded by ten CAG codons followed by one CAA codon and two additional CAG codons [9]. This CAG trinucleotide stretch is not found in other eukaryotic POLG genes with the exception of the African ...
Activated elF4B unwinds and linearizes such UTRs, allowing initial binding of the 40S ribosome and scanning towards the translation initiation codon24,25. Concurrently mTOR phosphorylates 4E-BP1, an inhibitory regulator of elF4E, leading to dissociation of 4E-BP1 from the cap complex, thereby ...
(Kozak, 1987)" CDS 1777..2496 /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ...
Therefore, we analyzed the codon usage values in HTT and ATXN3 transcript sequences upstream of the CAG repeat tracts (Fig. S3), but no significant differences were found for these mRNAs in this aspect (Supplementary Text). A2 and siHTT caused a decrease in HTT mRNA in the ...
Congenital isolated thyroid-stimulating hormone (TSH) deficiency is an autosomal recessive disease that manifests as hypothyroidism (cretinism), causing severe mental and growth retardations. Patients were found to have a single base substitution in the codon for the 29th amino acid of the TSH beta...
Alpha+-thalassemia trait caused by a nonsense mutation in the alpha2-globin gene: codon 54 (CAG>TAG). 来自 国家科技图书文献中心 喜欢 0 阅读量: 134 作者:B Eng,L Walker,JS Waye 摘要:We report a new alpha-thalassemia (alpha-thal) point mutation detected in a woman with alpha(+)-thal ...
a, Schematic depicting CRISPR-Cas9 targeting of exon 2 of FAN1 in Q109-n1 and Q109-n5 using two guide RNAs (gRNAs) to induce a 94 bp deletion leading to a premature stop codon and FAN1 knockout. The primer pair used for PCR screening of exon 2 after CRISPR is also shown. b, ...
Ready-to-use AAV2 particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA.CAG-driven tdTomato expression control. These AAV preparations are suitable purity for...