Modified single-stranded oligonucleotides were used to direct the nucleotide exchange of an A residue to a T residue in the second codon of the HD-gene, resulting in the creation of a leucine residue among the poly Q tract. Consistent with results from other groups, we provide evidence that ...
Near the N-terminus of the mature humanDNA polymeraseis a stretch of 13 glutamine residues encoded by ten CAG codons followed by one CAA codon and two additional CAG codons[9]. This CAGtrinucleotidestretch is not found in other eukaryoticPOLGgenes with the exception of the African great apes,...
The 72 × Gln, 72 × Ser, and 72 × Ala plasmids express C-terminal GFP-fused polypeptides with optimized codon after the ATG start codon. The expression was evident in cells transfected with the 72 × Gln, 72 × Ser, and 72 × Ala plasmids, but undetectable...
Product Category: Pre-made recombinant lentivirus; LV-CAG-hGluc-Puro; LV-CBA-hGluc-Puro; LV-CAG-Gluc-IRES-Puro; LV-CAG-hGluc-IRES-Puro; CAG-hGluc-Puro Lentivirus Description: LV-CAG-hGluc-Puro is a pre-made lentivirus which over-expresses codon-optimized/humanized Gaussia luciferase (h...
Activated elF4B unwinds and linearizes such UTRs, allowing initial binding of the 40S ribosome and scanning towards the translation initiation codon24,25. Concurrently mTOR phosphorylates 4E-BP1, an inhibitory regulator of elF4E, leading to dissociation of 4E-BP1 from the cap complex, thereby ...
First, please define the coding sequence of interest which holds the splice donor in question. The first nucleotide of the entered sequence must be the first nucleotide of a codon, in order to correctly read the encoded amino acid sequence. ...
(3858..3878) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4043..4178 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4105..4124 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4223..5017 /codon_start=1 /product...
In some Huntington disease (HD) patients, the “loss of interruption” (LOI) variant eliminates an interrupting codon in the HTT CAG-repeat tract, which causes earlier age of onset (AOO). The magnitude of this effect is uncertain, since previous studies
( 1999 ) Interaction of an α +-thalassemia deletion with either a highly unstable α -globin variant ( α 2 , codon 59, GGC GAC) or a nondeletional α -thalassemia mutation (AATAAA AATAAG): comparison of ... J Traeger-Synodinos,A Metaxotou-Mavrommati,M Karagiorga,... - 《Hemoglo...
In the absence of an AUG codon, RAN polyQ and RAN polyA proteins were detected for constructs containing expanded CAG repeat tracts (61 and 115 CAG) (Figure 1(b) and (c)). We did not observe any specific signal from potential polyserine (polyS) proteins (Figure 1(d)). In the case ...