免疫荧光法检测Caco-2单层细胞及紧密连接蛋白(tight junction proteins,TJ)的分布及结构变化。ELISA法检测各组Caco-2细胞炎症因子(TNF-α、IL-1β与HMGB-1)的分泌水平。Western blotting法检测各组Caco-2细胞紧密连接蛋白(occludin、ZO-1、claudin-1与claudin-4)的表达变化。 结果LPS损伤后,与对照组相比,LPS损伤...
目的通过观察高浓度丁酸钠(sodium butyrate,NaB)(5 mmol/L,8 mmol/L)对单层Caco-2细胞肠屏障模型的作用,及该过程中对NF-κB通路的影响,旨在探讨高浓度NaB破坏单层Caco-2细胞肠屏障模型的可能分子机制。 方法使用不同浓度的NaB及雷公藤甲素(triptolide,TP)(特异性抑制NF-κB)50 nmol/L作用体外培养的Caco-2细胞...
ride(LPS),peptidoglycan(PGN),lipoteichoicacid (LTA)andlipopeptides,stimulatetheinnateim- munesystem.Thetoll-likereceptor(TLR) family in the intestinal epithelial cells provides pathogen recognition (Takeda et al. 2003), and intestinal Cytotechnology 43: 57–64, 2003. ...
GPR120 has been shown to inhibit NF-κB signalling induced by lipopolysaccharide (LPS) after binding to Toll-like receptor 4 (TLR4) on macrophages and adipocytes, due to binding of β-arrestin2 in complex with transforming growth factor-β-activated kinase-1 (TAK1) and TAK1 binding protein-...
ZO-2 in THP-1 cells migrated from the cytoplasm to cell membrane in response to stimulation by LPS (data not shown), suggesting that occludin and ZO-2 would play a role in cell-cell interactions and the subsequent migration of THP-1 between the Caco-2 cell monolayers. The signi?cance ...
The monolayer permeability to soluble ion changes after treating with LPS, and TNF α as indicated by the reduction of the TEER value. In addition, the immune competent caco-2/U937-based model allowed the investigating the role of the epithelial layer as a protection barrier to biological ...
小檗碱对内毒素刺激致 Caco-2 细胞间高通透性的保护作用 李鑫;王洪岩;迟程;徐有青 【期刊名称】《实用肝脏病杂志》 【年(卷),期】2018(021)001 【摘要】Objective The aims of this study is to determine whether berberine(BBR) has a protective effect on lipopolysaccharide(LPS)-induced intercellular high...
Proanthocyanidin dimers had the most potent effect on increasing tight junction protein expression. The addition of LPS to Caco-2 cells induced oxidative stress and inflammation. However, incubation with proanthocyanidin dimers prevented LPS-mediated oxidative stress, including the increase of SOD, HO-1...
Progesterone decreased NF-κB activation in response to LPS, and further decreased permeability in human primary colon tissues and Caco-2 cells through upregulating tight junction protein occludin expression in vitro. These results suggest that the increased levels of progesterone during pregnancy may ...
表3 辣木多肽对LPS处理Caco-2细胞TEER与FD-40透过度的影响Table 3 Effects of MOPP on TEER and FD-40 levels in LPS treated intestinal Caco-2 cells 图3 辣木多肽对LPS处理Caco-2细胞中细胞间紧密连接相关因子的mRNA转录水平的影响Fig.3 Effects of MOPP on tight junction related factors mRNA expression ...