These low Ca2+-binding affinities are ideal for detecting high concentrations of Ca2+ in the endoplasmic reticulum and neurons (), as well as for tracking Ca2+ flux kinetics. Fluo-5N has been found to be particularly useful for monitori...
A critical aspect when designing a multimodal single-cell experiment is the extent to which information of ‘cell state’ can be extracted with each new data modality. Three factors to guide this selection are (i) the timescale of fluctuations in gene expression in relation to the measured phen...
Coupling between the Ca2+ entry and the intracellular Ca2+ stored in CM and VSMCs is mainly mediated by stromal interaction molecule, STIM1, located in the endoplasmic reticulum and ORAI1 membrane protein. These proteins are the primary components of the calcium-release activated calcium (CRAC)...
A common feature of different activator receptors in NK cells is the downstream phosphorylation of PLCγ, which subsequently mediates the production of IP3, activation of IP3receptor channel in the endoplasmic reticulum, resulting in Ca2+depletion of this store, which is coupled to CRAC assembling in...
Indeed, it was shown that Ca2+ overload of skeletal muscle and heart cells is primarily based on a significant decrease in sarco/endoplasmic reticulum (SR) Ca2+ ATPase (SERCA) activity, because SERCA accounts for ≥70% of Ca2+ removal from the cytosol [18,19,26,27]. It is suggested ...
Ca2 þ diffusion alone will not be sufficient in this case given the high buffering capacity of the cytosol. It has been estimated that the effective range of free Ca2 þ ions in the cytosol is 0.1 mm before it is buffered16. As Ca2 þ levels rise from the resting levels of B100 ...
Using time-lapse imaging accompanied by immunostaining and molecular analyses, we found that postovulatory ageing affects the amount of Ca 2+ stored in the cell, expression of Ca 2+ pump SERCA2, amount of available ATP and distribution of endoplasmic reticulum and mitochondria in a manner often ...
Sculpting the Ca2+ signature may be expected to require modulation of both Ca2+ entry into the cytosol and of transporters affecting reuptake/efflux. Several lines of evidence, such as the increase in transcript levels of plant endoplasmic reticulum (ER) Ca2+-ATPases in tomato (20) and ...
Calcium ions (Ca2+), one of the most abundant metal elements, is available in most soils2,3. It is involved in the growth, development and the adaptation to the environment in plants. It is also one of the most important second messenger upon environmental stimulation4. Through the cytoplasm...
As an important second messenger in the cell, calcium ion is a bridge connecting endoplasmic reticulum stress and mitochondrial dyshomeostasis [13]. The abnormal accumulation of intracellular calcium ions in time and space will determine the fate of the cell. Recent studies have shown that SOCs ...