Holloszy, Effects of Ca2+ ionophore ionomycin on insulin-stimulated and basal glucose transport in muscle, Am. J. Physiol. Regul. Integr. Comp. Physiol. 268 (1995) R997-R1002.Lee, AD, Gulve, EA, Chen, M, Schluter, J, Holloszy, JO (1995) Effects of Ca2+ionophore ionomycin on insulin...
CsA dramatically reduced the staurosporine apoptotic effects on Ba/F3 cells, having no significant influence on ionomycin treatment. Thus, we can consider that the calcium ionophore ionomycin is not inducing MPTP opening and apoptosis of Ba/F3 cells, although is increasing [Ca2+]c and [Ca2+]mt....
Ca 2+ ionophores and the activation of human-blood platelets-the effects of ionomycin, beauvericin, lysocellin, virginiamycin-s, lasalocid-derivatives and MCN-4308 . Biochimica et Biophysica Acta , 598 , 575 – 587 .Massini, P. & Naf, U. Ca2+ ionophores and the activation of human ...
Stimulation with the Ca2+ ionophore ionomycin and the sarco-/endoplasmic reticulum Ca2+-ATPase (SERCA) inhibitor thapsigargin in the presence of extracellular EGTA, a Ca2+-chelating agent, revealed dysregulated intracellular Ca2+ homeostasis in fibroblasts of P1 (Supplementary Fig. 5A, B). When ...
Upon extracellular application of the Ca2+ ionophore ionomycin, however, the inacti- vation was slowed down considerably (151 ± 13.5 ms) and the peak outward current was increased about twofold (Fig. 1b,c, red). No change in peak current upon ionomycin application was ...
Ca2+ release in response to the receptor agonist fMet-Leu-Phe, the Ca(2+)-ATPase inhibitor thapsigargin, or the Ca2+ ionophore ionomycin was also unaffected by GTP gamma S or GDP beta S. In contrast, the activation of the Ca2+ influx pathway by fMet-Leu-Phe or by thapsigargin was ...
To this end, cells were permeabilized with digitonin (or treated with the Ca2+ ionophore ionomycin) while perfused with a Ca2+-free medium containing 0.5 mM EGTA, to reach Rmin and finally, with a medium containing no EGTA and 3 mM CaCl2 to reach Rmax. Representative traces of [Ca2...
In the GH4C1 strain of rat pituitary cells, ionomycin, a divalent cation ionophore, induces a rapid and transient spike in cytosolic free Ca2+ concentrations [( Ca2+]i) similar to that induced by the Ca2+-mobilizing hormone thyrotropin-releasing hormone (TRH). To test directly the hypothesis ...
This was achieved by incubating cells for 1 h at 4 1C in Krebs-Ringer buffer (KRB) supplemented with 5 mM coelenterazine, 5 mM Ca2 þ ionophore ionomycin (Sigma-Aldrich) and 600 mM EGTA. After this incubation, cells were extensively washed with KRB supplemented with 2% bovine serum ...
Ca2 þ influx through the ORAI1 channel is often conveniently triggered through passive store depletion with thapsigargin (TG) or the Ca2 þ ionophore ionomycin in model cellular systems such as HeLa or HEK293 cells16. However, mutations causing loss of Ca2 þ -sensing capability (for ...