Heparin and Ca 2+ -free medium can enhance release of bull sperm attached to oviductal epithelial cell monolayers. Theriogenology 2001 . 56: 247 – 260 .Bosch, P., J. M. de Avila, J. E. Ellington, and R. W. Wright. Heparin and Ca 2+ -free medium can enhance release of bull ...
Furthermore, αLA-mediated GLP-1 secretion by STC-1 cells was significantly inhibited by Ca2+- free medium, implying that Ca2+ influx drives FFA4-mediated GLP-1 secretion[13]. However, nifedipine (an L-type Ca2+ chan- nel blocker), thapsigargin, and U-73122 (a phospholipase C inhibitor...
rich environments in the human body and in the culture - for example, 45–86 nM in the RBC cytosol, ~40 μM in the PV space, and ~1 mM in the human plasma25,26 - we fur- ther evaluated the effect of CPA and TG in Ca2+-free medium after selective membrane permeabilization...
2. 2. The response to caffeine was rapidly reduced after incubation in a Ca2+-free medium and was abolished after 6 min of incubation. However, the response to caffeine in Ca2+-free medium 30 sec. after pretreatment with 10 mM K+ in normal Ca medium was larger. 3. 3. These results ...
4B). Using immunofluorescence, we confirmed that both EDTA and Ca2+-free medium inhibited Nrf2 nuclear translocation (Fig. 5E). It is worth noting that the ratio of Nrf2-positive cells in the nucleus in the EDTA-treated and Ca2+-free group were similar to the control group, suggesting that...
The effect of ET-1 persisted when Ca2+ was removed from superfusion medium (mean Δ[Ca2+]i in Ca2+-free medium, 233 ± 40 nmol/L above basal, N = 8, Figure 1d, right). Download: Download full-size image Figure 1. Dose-dependent [Ca2+]i increases induced by basolateral superfusion...
Rat neonatal ventricular myocytes were cultured in the standard serum-free medium (no insulin) for 48 h. This resulted in Ͼ90% of the cardiomyocytes being arrested in the G0-G1 phase. After this process, cells were incubated in the same medium as for electrophysiologic studies, supple- ...
(1998), Functional Ca2+ and Na+ channels on mouse Schwann cells cultured in serum-free medium: regulation by a diffusible factor from neurons and by cAMP. European Journal of Neuroscience, 10: 1796–1809. doi: 10.1046/j.1460-9568.1998.00193.x Author Information INSERM U394, I.F.R.8, ...
The first objective of the present study was to determine if Ca2+/Mg2+ free medium is indispensable for aspiration of blastomere from every stage of day-3 8-cell embryos. The second objective was to evaluate whether biopsy procedures with or without Ca2+/Mg2+ free medium affected subsequent ...
Preincubation of day-3 embryos in Ca2+/Mg2+-free medium is a routine procedure to loosen interblastomere connections before aspiration regardless of their stages before or after compaction. The first objective of the present study was to determine if Ca2+/Mg2+ free medium is indispensable for...