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Patch pipettes were prepared using a P-97 puller (Sutter Instruments, Novato, CA, USA) and were fire-polished to a final resistance of 2–3 MΩ. To study the voltage dependence of channel activation, a single-step voltage protocol was used: depolarizing test pulses with 1-s durations ...
The final assembled full-length cDNAs were cut with HindIII/XbaI and subcloned into pcDNA3-HE3 downstream of the 5′ -UTR from the Xenopus laevis β-globin gene to improve expression in Xenopus oocytes. Chemicals and preparation of solutions. Most of the chemicals for electrophysiological...
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ICAI has come up with notification on 24th April 2023, introducing a new pattern of 30:70 assessment for few objective papers of IPCC syllabus for May 2023 examination. For May 2023 preparation, Students can download LatestIPCC study materialandIPCC practice manual,IPCC RTPandIPCC Question papers...