C2C12 cellsAtrogin-1MuRF1Background Sarcopenia is a disease of progressive loss of muscle mass due to the imbalance of protein synthesis and proteolysis, and tends to emerge with ageing. Currently its treatment consists of non-drug therapies and drug therapies, but some medications can have ...
To explore the mechanism of SPARCL1 regulation of muscle differentiation in mice, a muscle injury mice model was used in this study. We also activated and inhibited the expression of SPARCL1 to examine its influence on C2C12 cells differentiation. Furthermore, co-immunoprecipitation (Co-IP) and ...
生长特性：C2C12是一种贴壁型细胞系。传代建议：C2C12播种密度保持在1 x 10⁴ cells/cm²。在这种播种密度下，细胞大约需要4天形成一层密集的单层。播种细胞时，使用磷酸盐缓冲溶液（PBS）冲洗细胞，并将其与传代溶液（Accutase）孵育。随后，离心收集脱落的细胞并重悬于生长培养基中。将重悬的细胞倒入新的培养瓶...
生长特性:C2C12是一种贴壁型细胞系。 传代建议:C2C12播种密度保持在1 x 10⁴ cells/cm²。在这种播种密度下,细胞大约需要4天形成一层密集的单层。播种细胞时,使用磷酸盐缓冲溶液(PBS)冲洗细胞,并将其与传代溶液(Accutase)孵育。随后,离心收集脱落的细胞并重悬于生长培养基中。将重悬的细胞倒入新的培养瓶中进行...
传代建议:C2C12播种密度保持在1 x 10⁴ cells/cm²。在这种播种密度下,细胞大约需要4天形成一层密集的单层。播种细胞时,使用磷酸盐缓冲溶液(PBS)冲洗细胞,并将其与传代溶液(Accutase)孵育。随后,离心收集脱落的细胞并重悬于生长培养基中。将重悬的细胞倒入新的培养瓶中进行培养。
传代建议:C2C12播种密度保持在1 x 10⁴ cells/cm²。在这种播种密度下,细胞大约需要4天形成一层密集的单层。播种细胞时,使用磷酸盐缓冲溶液(PBS)冲洗细胞,并将其与传代溶液(Accutase)孵育。随后,离心收集脱落的细胞并重悬于生长培养基中。将重悬的细胞倒入新的培养瓶中进行培养。
C2C12 mouse myoblast cell line was used for the in-vitro studies, and MTS assay and fluorescence microscopy were used to study the cell viability, proliferation, and differentiation. X-ray diffraction and Energy Dispersive X-ray results confirmed the chemical structure of a uniform amorphous SiONx...
Moderate hypoxia promotes skeletal muscle cell growth and hypertrophy in C2C12 cellsProtein expressionsMuscle cell differentiationMyogenesisResistance exerciseSarcopeniaAlthough several studies have implied that a hypoxic environment may be a factor that influences muscle hypertrophy, scant attention has been paid...
Isrn Stem CellsKhayat G, Rosenzweig DH, Khavandgar Z, Li J, Murshed M, Quinn TM: Low-frequency mechanical stimulation modulates osteogenic differentiation of C2C12 cells. ISRN Stem Cells 2013, 2013:138704.Khayat G, Rosenzweig DH, Khavandgar Z, Li J, Murshed M, Quinn TM: Low-frequency ...
原名:Comprehensive Analysis of the Proteome and PTMomes of C2C12 Myoblasts Reveals that Sialylation Plays a Role in the Differentiation of Skeletal Muscle Cells 译名:C2C12成肌细胞蛋白质组和PTMomes的综合分析表明唾液酸化在骨骼...