Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ma...
Cell StiffnessAFM Force Mapping.BMP-2 is known to play a major role in osteogenic differentiation of various cell types by interacting with serine/threonine-specific kinase receptors and up- or down-regulating intracellular signaling cascades via SMADs proteins. C2C12 myoblast cells were stimulated by...
Every step of the way, a wide range of cell health products Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell bio...
157 ROB-C26 cells, a rat calvaria-derived multipotential cell line, are known to differentiate into osteoblasts, muscle cells, and adipocytes by various stimuli. When cultured with BMP-2 or BMP-6/Vgr-1, the ROB-C26 cells differentiated into cells of the osteoblast phenotype.34,165 BMP-2 ...
C2C12 cells were treated with a range of 3-MPA doses (0–1 mM) and total DNA content quantified as a marker of total cell number. There was a significant time × treatment interaction (P < 0.001), with 3-MPA inducing a dose-dependent (0.1–1 mM) reduction in total DNA con...
Full size image SPARCL1 regulates BMP7 expression and BMP/TGF-β cell signaling pathway To verify the interaction between SPARCL1 and BMP7, SPARCL1 was activated or inhibited with CRISPR/Cas9 or siRNA, respectively. The expression of BMP7 was then detected by western blotting. The results showe...
Cell fusion, with nuclei aggregation in the center, can be observed using methods such as Image J software, Giemsa staining, and phase contrast microscopy, resulting in the formation of immature myotubes and even myofibers. However, the C2C12 cell line has a limited lifespan and can only be ...
c2c12
2) Compared with the con⁃ trol group, t10,c12⁃CLA inhibited the myogenic differentiation of C2C12 myoblasts, significantly decreased the intracellular myotube numbers / cell numbers and down⁃regulated intracellular genes expression levels of myogenin ( MYOG) and myogenic differentiation antigen ...
weobservethecellappearanceandfindthatcellsprocessedbyPAandOAhasthetendencytogrowinonedirectionwithouteellfusionorwithonlytwocellfusion.Meanwhile,thepolymorphonuclearmyotubedoesnotarise.InthecytoplasmcellsappearonbothendsofdifferentsizelargeLAprocessing,lipiddropsinthecytoplasmandcell,smallnumberoflipiddropsscatteredlipid...