Shu Ye Jiang,Minnie Cai,Srinivasan Ramachandran.The Oryza sativa no pollen (Osnop) gene plays a role in male gametophyte development and most likely encodes a C2-GRAM domain-containing protein[J]. Plant Molecular Biology .2005(6)Jiang,S.Y,et al.The Oryza sativa no pollen (Osnop) gene ...
Protein A 保存液 PBS 内含物 0.02% sodium azide 保存条件 -20°C 运输条件 Ambient (domestic); Wet ice (international) RRID AB_11153821 产品详细信息 A suggested positive control is EL4 cell lysate. 靶标信息 Myeloid differentiation Marker 88 (MyD88) is an adaptor molecule for the Toll/IL-1R ...
All EF hand structure domains are structurally related and members of the same clan [3]. EF hand structures occur in other proteins, notably the ciliary protein EFCAB7 (“EF-hand calcium-binding domain-containing protein 7”) which has been shown to be a positive regulator of hedgehog ...
Chemical complexity is vital not only for the origin of life but also for biological evolution. The chemical evolution of a complex prebiotic mixture containing acetylene, carbon monoxide (CO), and nickel sulfide (NiS) has been analyzed with mass spectro
(Supplementary Fig.1). As Jovi-1 showed optimal TRBC1 binding specificity, we use knowledge of the epitope contact interface and binding angle for the generation of a TRBC2 binder using structure-guided computational biology. This approach is challenging from a protein engineering perspective and ...
The vectors containing MAP, the target gene (pcsk2orkex2) and BmT1 homologous region were constructed to investigate the availability of counter-selection (Fig.4). To avoid the influence of leakage toxicity of the promoter on the counter-selectable marker such as MazF, an appropriate promoter sh...
The cells were subsequently serially diluted, and 3 μl of the cell suspension were spotted on anaerobic medium plates containing 0.2% arabinose (w/v) or 50 μm IPTG and 0.5% glucose, respectively. The anaerobic growth was carried out in sealed bottles under O2-free environment. Protein ...
摘要: The aim of this study is to link the variation in muscle physiological responses to disuse atrophy (a "non-pathological" but commonly debilitating form of muscle wasting) with variation in protein …" [more]DOI: 10.1016/S0168-8278(09)60769-8 年份: 2009 ...
(Supplementary Fig.5b), suggesting that our protocol gives rise to incomplete reconstitution and possibly a fraction of [3Fe–4S] clusters.26IseH exhibited a typical UV-Vis spectrum for a [4Fe–4S] cluster-containing protein (Supplementary Fig.6). Like other radical SAM enzymes, IseH cleaved...
After 48 h, the cells were lysed using NP40 buffer containing protease inhibitors. Total proteins were quantified using a PierceTM Coomassie (Bradford) Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and separated by electrophoresis on 10% SDS-polyacrylamide gel (Biorad, Hercules,...