C-terminal peptide and protein sequencingMethodology involving novel coupling reagents for the carboxyl- terminal amino acid sequence analysis of peptides and proteins is described.Chad G. Miller
C-terminal peptide and protein sequencing 来自 FreePatentsOnline 喜欢 0 阅读量: 10 申请(专利)号: 07/271328 申请日期: 11/15/1988 公开/公告号: US4935494 公开/公告日期: 06/19/1990 发明人: CG Miller 被引量: 34 摘要: Methodology involving novel coupling reagents for the carboxyl-...
专利名称：C-terminal peptide or protein sequencing reagent and method 发明人：Jerome M. Bailey,John E. Shively 申请号：US07/801944 申请日：19911203 公开号：US05180807A 公开日：19930119 专利内容由知识产权出版社提供 摘要：A carboxy terminal protein sequencing process is disclosed which utilizes ...
组成蛋白质的多肽链不是闭合的,而是链状的化合物,含有两个末端,即氨基端(N端)和羧基端(C端)。大量研究表明蛋白质的C端和N端有着不同的重要作用:蛋白质N端序列与蛋白质的寿命和亚细胞器定位等有密切关系;C端序列影响蛋白质和多肽的空间结构和生物功能。对蛋白的末端序列进行研究有利于蛋白质的高级结构分析,从...
a, N-terminal sequencing of the purified NLRP1–DPP9 complex, showing that the NLRP1 CT is not cleaved by the co-expressed DPP9. b, Chemical enrichment of protease substrates assay, showing that DPP9 does not cleave NLRP1 CT. In brief, wild-type NLRP1 expressed in DPP8 DPP9 double-...
Mouse WB, IP, IF, IHC, ICC His (C-terminal Specific)-tagged protein in all species - - WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing. ...
(2001)0120058204 C2terminalSequencingofProteinandPeptidebyMALDI2TOFMassSpectrometry CHENPing,LIANGSong2ping (DepartmentofBiochemistryandMoleculanBiology,HunanNormalUniversity,Changsha410081,China) Abstract:Usingofmatrix2assistedlaserdesorptionΠionizationtime2of2flight(MALDI2TOF)massspectrometrycou2 pledwith...
Since the introduction of the solid-phase approach to N-terminal protein sequencing by Laursen, R. A. J. Amer. Chem. Soc., 88:5344-5346 (1966), several different types of functionalized supports have been described for the covalent immobilization of polypeptide samples. These include polystyrene...
1) C terminal sequencing 蛋白质C端序列分析 2) protein sequence analysis 蛋白质序列分析 1. To find the functional similarity of proteins with low identity is a hinge ofprotein sequence analysis. 对于蛋白质序列分析,关键在于有效地发现相似的序列片断、特定的结构—功能域和低相似性的同源序列。
5) protein N-terminal sequencing 蛋白N端测序 6) C-terminal sequencing C端测序 1. The principles,characteristics,applications and new development in proteomic analysis of threeC-terminal sequencingmethods including carboxypeptidase method,chemical method and tandem mass spectrometry method were introduced in...