Original article http://dx.doi.org/10.6065/apem.2015.20.2.92 Ann Pediatr Endocrinol Metab 2015;20:92-97 Preserved C-peptide levels in overweight or obese compared with underweight children upon diagnosis of type 1 diabetes mellitus Hyeoh Won Yu, MD, Yun Jeong Lee, MD, W...
The palmitoylation of cysteine 23 residue (C23) in PHF2 was identified based on the shift of peptide peaks. d After transfection with wild-type (WT) PHF2 or C23A-mutated PHF2 plasmids, HCC cells lysates were subjected to western blotting. n = 3 independent experiments. e HCC cells ...
The above results suggested that YAP signalling was indeed activated in cells with low ABHD5 expression; to corroborate this, we measured YAP nuclear accumulation in ABHD5-knockdown HCT116 cells and found that the levels were higher compared to those in control cells (Fig. 4c, d). Accordingly...
We reanalysed public deep proteome profiles of healthy human donors for signatures of CTAP formation. If CTAPs arise from α-amidating cleavage, we would expect to detect peptides with C-terminal amidation (−0.984 Da) together with a neo-C-terminus (not the annotated protein C terminus...
The plasma level of HBP, procalcitonin (PCT) and C-reactive protein (CRP) were measured, and the detailed clinical data were retrieved from the patient chart records for all patients described above. Comparison of each laboratory and clinical parameters between groups was carried out by Non-...
497, 1993. Eipper BA, Stoffers DA, Mains RE: The biosynthesis of neuropeptides: peptide alpha...
In turn, PAL catalyzes the conversion of peptidyl α-hydroxylglycine to an amidated peptide (and glyoxylate).Amidation of an antibody can be controlled by altering certain conditions during the cell culture process. For example, copper (e.g., in ferric ammonium citrate) and/or oxygen levels ...
For further statistical analyses, the acquired peak areas were exported at the fragment, peptide, and protein levels. To determine significantly up-or down-regulated proteins of C. jejuni 81–176 in the different temperature conditions, the Empirical Bayes method for mixed models implemented in the...
The study found that oral collagen peptides demonstrably enhanced skin elasticity, smoothness, and dermis echo density, while proving safe and well-tolerated by participants.A noteworthy improvement in skin elasticity, the alleviation of roughness, and an increase in dermis echo density was observed ...
Peptide samples were analyzed on the nanoElute coupled online with a timsTOF Pro mass spectrometer (Bruker). Peptides were re-dissolved in 0.1% formic acid (FA) and loaded onto a 25 cm in-house column n (360 μm OD × 75 μm inner diameter) packed with C18 resin (particle size ...