Anti-c-Myc antibody [Y69] is a rabbit monoclonal antibody used for detecting c-Myc, a transcription factor involved in cell proliferation, growth, and differentiation. Validated for western blotting (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), immunocytochemistry/immunofluorescence ...
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Afterwards, chromatin was fragmented by sonication and incubated overnight at 4 °C with anti-c-Myc antibody (Abcam, ab32072) or IgG (Proteintech, B900610) and Protein A/G magnetic beads. After washing the complexes with low-salt and high-salt solutions in turn, purified DNA was ...
The co-expression of CFP and transthyretin in scat- tered epithelial cells of the postnatal mouse CP in Nestin-CFP mice [12] suggested that our construct was expressed in the epithelial lining of the CP (Add- itional file 3: Figure S2G). To confirm that it was c-MYC overexpression in ...
The following antibodies were used for western blot: c-Myc (Abcam, ab32072, 1:1000), Wnt2b (Abcam, ab178418, 1:1000), beta Catenin (Abcam, ab68183, 1:1000), E-cadherin (Abcam, ab40772, 1:1000), α-catenin (Abcam, ab51032, 1:1000), N-cadherin (Abcam, ab76011, 1:1000), ...
c-MYC IHC analysis was performed using an antibody against c-MYC (clone Y69, catalog ab32072, Abcam, Burlingame, CA, USA). ß-catenin IHC used a commercially available antibody against ß-catenin (clone CAT-5H10, Invitrogen, Camarillo, CA, USA). The staining process was performed using...
Anti-CAMKIIγ (8G10C1) (IHC) Abcam Cat# ab201966 Anti-p-CAMKIIγ Santa Cruz Biotechnology Cat# sc-12886-R; RRID: AB_2067915 Anti-c-Myc (Y69) Abcam Cat# ab32072; RRID: AB_731658 Anti-TRIM24 Novus Cat# NB100-2597; RRID: AB_10002487 Anti-GAPDH Cell Signaling Technology Cat# 2118...
结论AZA联合HHT通过激活ISR信号通路,调控c-MYC/DDIT3/PUMA轴,抑制细胞增殖和促进细胞凋亡,发挥协同抗AML作用。 关键词: 阿扎胞苷;高三尖杉酯碱;白血病,髓系,急性;协同效应 ABSTRACT ObjectiveThis study aimed to explore the synergistic effect and underlying mechanism of azacitidine (AZA) in combination with ...
Soluble proteins were analyzed by immunoblotting with anti-MUC1-C (Thermo Scientific), anti-MYC (Abcam; AB32072 reactive with MYC and not MYCN or MYCL), anti-β-catenin (BD Biosciences), and anti-β-actin (Sigma) antibodies. Detection of immune complexes was achieved using horseradish ...
(Roche Diagnostics)]. The following antibodies recognizing human proteins were used: Anti-c-Myc rabbit monoclonal (cat. no. ab32072; 1:10,000; Abcam), anti-puromycin mouse monoclonal (cat. no. MABE343; 1:10,000; Merck KGaA), anti-GS mouse monoclonal (cat. no. 610517; 1:250; BD ...