線虫(Caenorhabditis elegans)は、印象的な走化性行動を持っています。 着臭剤のワームの応答を試験後ろ前提領域に置き、臭剤に応答誘発動きを観察することである。でも、多くの利用可能なアッセイで、有意なサンプルサイズを維持しながら、互いにワームの相互作用を最小限に抑えながら、対照お...
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This protocol outlines the isolation of membrane protein complexes from transgenic C. elegans The primary advantage of the protocol is that it enables isolation of sufficient quantities of low-abundance native membrane protein complexes for use in structural, biochemical or biophysical studies This is a...
Animals increase by orders of magnitude in volume during development. Therefore, small variations in growth rates among individuals could amplify to a large heterogeneity in size. By live imaging of C. elegans, we show that amplification of size heteroge
saprykin/plibsys - Highly portable C system library: threads and synchronization primitives, sockets (TCP, UDP, SCTP), IPv4 and IPv6, IPC, hash functions (MD5, SHA-1, SHA-2, SHA-3, GOST), binary trees (RB, AVL) and more. Native code performance. eudoxia0/magma - Extending C with ...
saprykin/plibsys - Highly portable C system library: threads and synchronization primitives, sockets (TCP, UDP, SCTP), IPv4 and IPv6, IPC, hash functions (MD5, SHA-1, SHA-2, SHA-3, GOST), binary trees (RB, AVL) and more. Native code performance. mellowcandle/bitwise - Terminal based...
The protocol requires 10 d for worm growth, liquid culture and synchronization; 1-2 d for bio-orthogonal labeling; and, with regard to analysis, 3-4 d for western blotting, 5-6 d for immunofluorescence or ~3 weeks for mass spectrometry....
for example, a recent report using the nematodecaenorhabditis elegansshowed that the sublethal concentrations of silver nanoparticles, which are extensively used in consumer products and biomedical applications, alter the development, behavior, and mitochondrial function in worms that harbor genetic defects ...
elegans. However, the mechanism whereby G3PP activation extends healthspan and lifespan, particularly under glucotoxicity, remained unknown. Here, we show that the overexpression of the C. elegans G3PP homolog, PGPH-2, decreases fat levels and mimics, in part, the beneficial effects of calorie...
Protocol 1. 径向运动测定 准备直径为100毫米或150毫米的培养皿,大约一半充满线虫生长培养基(NGM),并用均匀的OP50细菌草坪播种以覆盖琼脂的整个表面。 将NGM测定板倒置,并在底部标记要测定的秀丽隐杆线虫菌株的标识符,每个菌株2个板(图1A)。 用倒置板中心的标记做一个小点(图1A)。