Clinical Outcome of C. difficile-Toxin Negative, PCR-Positive PatientsUsmani, AsharBoyce, JohnThe American Journal of Gastroenterology
实时荧光定量PCR(quantitative real-time PCR,qPCR)是指在PCR反应体系中加入荧光基团,利用荧光信号积累实时监测整个PCR进程,*通过标准曲线对未知模板进行定量分析的方法。实时荧光定量PCR的化学原理包括探针类和非探针类两类,探针类是利用与靶细胞序列特异性杂交的探针来指示扩增产物的增加,非探针类是利用荧光染料或者特异...
英文名称 Anti-TNFC/lymphotoxin Beta 中文名称 C/淋巴毒素β抗体 浓 度 1mg/1ml 规 格 0.1ml/100μg 0.2ml/200μg 抗体来源 Rabbit 克隆类型 polyclonal 交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Rabbit 产品类型 一抗 别 名 LT beta; LTB; lymphotoxin beta (TNF superfamily, member 3); ...
The goal of this study was to compare the outcome and efficiency of PCR assays, BD GeneOhm and GeneXpert, for detecting C. difficile toxin B gene in samples that have tested indeterminate for C. difficile by the Quik Chek Complete test. Over a three-month period, a total of 41 cases ...
Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 BD GeneOhm Cdiff. BD Becton Dickinson. Rupnik, M et al. A novel toxino...
The multiplex PCRBioFire®FilmArray®Gastrointestinal (GI) Panelidentifies 22 pathogens commonly associated with gastrointestinal infections, includingC. difficile, with results in about an hour. The BioFire GI Panel’sC. difficileassay targets both the toxin A and toxin B genes. Overall, the pane...
In addition to toxin degradation and strain differences, we hypothesized that C. difficile concentration could be important in determining whether toxins are detected in fecal samples. We performed standard curves on an FDA-approved real-time PCR test for the C. difficile tcd B gene (Xpert C. ...
Two clostridial toxins primarily mediate pathogenicity: toxin A and toxin B [1]. Of note, some strains such as ribotype 027 (RT027) may express an additional toxin (CDT, binary toxin, gene: cdtAB) which may lead to more severe courses of disease. RT027 has also been the cause for ...
(noC. diff, or uninfected mice). Mutation oftcdRhas been reported to significantly reduce levels of both toxin gene expression and toxin protein production in vitro34,35. Nearly five-fold fewertcdRvegetative cells were recovered in the feces relative to wild type at day 1 (p = 0.0314,...
Clostridium difficile is the leading cause of nosocomial diarrhea and colitis in the industrialized world. Disruption of the protective gut microbiota by antibiotics enables colonization by multidrug-resistant C. difficile, which secrete up to three diff