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And for nuclear and cytoplasmic lysates, cells were extracted via Nuclear and Cytoplasmic Protein Extraction Kit (KeyGEN BioTECH, Shanghai, China) according to the manufacturer's instructions. Protein concentrations were measured using a Bicinchoninic Acid Assay Kit (BCA, Thermo Fisher Scientific, Inc....
See Supplementary Data 3 for statistical analysis and replicate data. Construction of cct C. elegans expression plasmids To construct the cct C. elegans expression plasmids, pPD95.77 from the Fire Lab kit was digested with SphI and XmaI to insert 3.6KB of the sur5 promoter. The resultant ...
Bands were automatically detected and quantified with Image LabTM Software (Biorad). qPCR. RNA was extracted using the Nucleospin Triprep kit (Macherey-Nagel). After quality con- trol with the Bioanalyser (Agilent), cDNA synthesis was executed (Tetro cDNA synthesis kit, Bioline) fol- lowed...
//answers.microsoft.com/en-us/windows/forum/all/sign-in-to-games-for-windows-live-error-this-page/0a82e2ce-0880-4fc7-8e15-bca7b5425eb6 2018-02-27T18:52:50.0000000Z https://answers.microsoft.com/en-us/msoffice/forum/all/when-trying-to-run-the-scanpstexe-utility-to-clean/aa79740a...
https://pentestlab.blog/category/red-team/ -72-The Ultimate Penetration Testing Command Cheat Sheet for Linux: https://www.hackingloops.com/command-cheat-sheet-for-linux/ -73-Web Application Penetration Testing Cheat Sheet: https://jdow.io/blog/2018/03/18/web-application-penetration-testing...
Programs are - // currently owned by the compilation cache which means we must wait for - // program completion in the destructor. There are multiple compilation caches - // around, which complicates things a little. Perhaps having programs be - // shared_ptrs (an invasive change) would ...
For quantitation, densitometric values of the phosphorylated protein were normalized to the amount of immunoblotted OATP2B1. Time-lapse Analyses OATP2B1-GFP-expressing cells were seeded into LabTekIITM chambered coverglass (Nunc, Wiesbaden, Germany) and incubated in normal tissue culture medium in a...
The levels of nuclear protein Lamin A/C are crucial for nuclear mechanotransduction. Lamin A/C levels are known to scale with tissue stiffness and extracellular matrix levels in mesenchymal tissues. But in epithelial tissues, where cells lack a strong in
(Fig.2b) were confirmed for the duration of the CAM assay until 168 h (day 7). After seedinglincNMR-silenced or control-treated cells onto the CAM, tumors were harvested, measured, and weighed. Tumors derived fromlincNMR-depleted cells were significantly smaller in size than tumors ...