To generate the siRNA-resistant plasmid of NDR1, three bases of targeted sequence were mutated by Quickchange site-directed mutagenesis kit (Agilent Technologies): sr-NDR1 Scientific Reports | 5:10449 | DOI: 10.1038/srep10449 10 www.nature.com/scientificreports/ Figure 6. Working model ...
Liver-specific NDRG1 KO male mice were obtained using CRISPR/Cas9-based adeno-associated virus-8 (AAV-8; catalog GV487, GeneChem Technologies, sgRNA: CATGCCGATGTCGTGATACGT). The establishment of NDRG1 KO mice was confirmed by PCR or Western blot analysis. For overexpression, NDRG1-KO mice...
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