2Department of Marine Electronics, Gdynia Maritime University, Morska 81‑87, 81‑225 Gdynia, Poland. *email: bhrycak@ imp.gda.pl Scientific Reports | (2023) 13:2204 | https://doi.org/10.1038/s41598-023-29433-y 1 Vol.:(0123456789) www.nature.com/scientificreports/ Many ...
Real-time reverse transcription polymerase chain reaction (RT-PCR) RNA was isolated from the 8305C cells using the Total RNA Isolation kit (A&A Biotechnology, Gdynia, Poland) accord- ing to the manufacturer's instructions. First strand cDNAs were obtained by reverse transcription of 1 μg of ...
Total DNA was extracted using Genomic Mini AX Bacteria kit (A&A Biotechnology, Gdynia, Poland) according to the manufacturer’s protocol. For soil samples, additional mechanical lysis with zirconium beads was performed using FastPrep-24 instrument (MP Biomedicals). After isolation, the DNA from eac...