The following electroporation program was used: square-wave protocol with either 250 V for iPS or 300 V for MEF cells, each 10 ms pulse length, 2 pulses, 10 s pulse interval, and 4 mm cuvette. (a) Transfection efficiency. Transfected cells for mCherry and Venus are depicted by red and...
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Primary production by phytoplankton mediates about half the global carbon and nitrogen cycles, despite the rarity of favourable conditions for their growth in the surface ocean. Nutrients are typically scarce and light is only plentiful above the chemocline depth, which varies from 60–180 m. Meanwh...
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(red, high; white, low).cExamples of other large deletions detected by clumps; plots show AFs.dMutation inTP53(tumor protein 53) that is identified as a clump in the tumor cells. The smoothed AF is shown on the right, and the RD for each cell is shown below.eSNVs in theTCTN3(...
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