本发明公开了重组抗菌肽buforin IIb的生产方法,应用重组DNA技术,构建pSUMO/buforin IIb原核表达载体,再转化大肠杆菌,构建表达工程菌;工程菌以乳糖为诱导剂诱导表达SUMO-buforin IIb融合蛋白;然后经细胞破碎后释放出融合蛋白,使用镍离子亲和层析纯化,纯化后的融合蛋白经SUMO酶切反应,释放出buforin IIb,经镍离子亲和层析...
我们提供 产品名称:Buforin IIb;RAGLQFPVG[RLLR]3 ;穿膜肽品牌:上海楚肽生物科技有限公司产地:上海价格:面议021-60871011 产品名称:Buforin II;TRSSRAGLQFPVGRVIIRLLRK;穿膜肽品牌:上海楚肽生物科技有限公司产地:上海价格:面议021-60871011 更多信息请联系我们详细咨询联系电话:17321195801 021-60871011 QQ:840363522...
本发明公开了重组抗菌肽buforinIIb的生产方法,应用重组DNA技术,构建pSUMO/buforinIIb原核表达载体,再转化大肠杆菌,构建表达工程菌;工程菌以乳糖为诱导剂诱导表达SUMO-buforinIIb融合蛋白;然后经细胞破碎后释放出融合蛋白,使用镍离子亲和层析纯化,纯化后的融合蛋白经SUMO酶切反应,释放出buforinIIb,经镍离子亲和层析分离得到...
本发明公开了重组抗菌肽buforin?IIb的生产方法,应用重组DNA技术,构建pSUMO/buforin?IIb原核表达载体,再转化大肠杆菌,构建表达工程菌;工程菌以乳糖为诱导剂诱导表达SUMO-buforin?IIb融合蛋白;然后经细胞破碎后释放出融合蛋白,使用镍离子亲和层析纯化,纯化后的融合蛋白经SUMO酶切反应,释放出buforin?IIb,经镍离子亲和层析...
However, the molecular mechanism of buforin IIb on prostate cancer cell has still not been determined. In this study, we found buforin IIb significantly inhibited the prostate cancer cells proliferation, Furthermore, buforin IIb-induced cell apoptosis through downregulation of pro-caspase 3/8/9, ...
The cell cycle was arrested at the G2/M phase following buforin IIb treatment. CDK2 and cyclin A were downregulated by treatment of the cells with 1.0M buforin IIb for 24h. Treatment with buforin IIb also inhibited the migration of liver cancer cells in vitro. Furthermore, 50nmol buforin ...
本发明提供了一种抗菌肽Buforin IIB的改造体镇痛肽BRL及其应用.所述镇痛肽BRL的氨基酸序列如SEQ ID NO.1所示,其是根据抗菌肽Buforin IIB的氨基酸序列经过改造获得的;该镇痛肽BRL具有显著的抑菌活性,镇痛活性和抗炎活性,且结构简单,获取方便.所述镇痛肽BRL能够应用于治疗或缓解疼痛的药物,或者治疗或缓解炎症的药物的...
在BuforinIlbC末端的两个仪螺旋和一个仪螺旋模型序列之间有一个脯氨酸铰链区,BufomIIb—a是buforinIIb的合成类似物,是具有潜在细胞渗透性的抗菌肽.为了开发新型具有高活性,高特异性及高疗效的抗菌肽,通过替换脯氨酸铰链区和buforinIIb两个a螺旋区的氨基酸,构建了若干类似物(BufIII类似物),并检测其抗菌活性和作用...
The method which enables the recombinant antibacterial peptides buforin IIb to be efficiently expressed in prokaryotic host cells has the advantages of simple separation and purification, high recombinant protein purity, easy operation, easy amplification, good expression product stability, and suitableness...
Buforin IIbOlaparibDNA damageImmunogenic cell deathCancer demonstrates an extreme resistance to induced DNA damage, resulting in treatment failure, recurrence, and unfavourable prognosis. This work found Buforin IIb peptide and the poly(ADP-ribose) polymerase (PARP) inhibitor Olaparib (OLA) as a novel...