缓冲液配方（BufferRecipe）Buffers N.B. Azide should not be added to any buffers that will be used directly with HRP-labeled antibodies because it will inactivate the enzyme.PBS 8.0 g NaCl 0.2 g KCl 1.15 g Na2 HPO4 0.2 g KH2 PO4 Dissolve in 800 ml distilled water, adjust pH to...
10× PCR buffer Recipe (GC-rich) 200 mM Tris-HCl (pH8.8 @25℃) 100 mM KCl 100 mM (NH4)2SO4 20 mM MgSO4 1% Triton X-100 1 mg/mL BSA Long PCR BufferA 5X buffer stock containing 6 mM Mg(OAc)2 (1.2 mM final concentration) and 5% DMSO (1% final concentration) can easily be ...
缓冲液配方(Buffer Recipe)缓冲液配方(buffer recipe) buffersn.b. azide should anybuffers useddirectly hrp-labeledantibodies because enzyme.pbs 8.0 nacl0.2 kcl1.15 800ml distilled water, adjust ph addmore distilled water finalvolume liter.sterilize roomtemperature (rt). tbs 8.0 nacl0.2 kcl3.0 tris...
缓冲液配方(Buffer Recipe) 热度: 各种缓冲液(buffer)知识 热度: PBS缓冲液的配方 热度: 相关推荐Buffers N.B.AzideshouldnotbeaddedtoanybuffersthatwillbeuseddirectlywithHRP-labeledantibodiesbecauseitwillinactivatetheenzyme. PBS 8.0gNaCl 0.2gKCl
Calculate compositionof any buffer in no time. Force ionic strength with any inert salt. Select buffers from thebuffer database, add buffers to the database, modify buffers in the database. Print an exact recipewith pH vs temperature and buffer capacity vs pH plots. ...
10×PCR buffer Recipe (GC-rich)200 mMTris-HCl (pH8.8 @25℃) 100 mMKCl 100 mM(NH4)2SO4 20 mMMgSO4 1%Triton X-100 1 mg/mLBSA Long PCR BufferA 5X buffer stock containing 6 mMMg(OAc)2 (1.2 mMfinalconcentration) and 5% DMSO (1% final concentration) can easily be made using 500 ...
Hybridization buffer recipe New forums postsCatalog, OnlineSpecial, SamplesElectrophoresis, Gel
50x TAE buffer recipe The recipe below can be used to prepare a 50x 1 L stock solution of TAE buffer. From this, a 1x working solution can be prepared. ReagentWeight/VolumeFinal concentration Tris base242 grams2 M Glacial acetic acid57.1 mL1 M ...
Recipe:150 mM NaCl 1.0% Nonidet P-40 (NP-40)50 mM Tris-Cl Adjust pH to 7.4 它可能是最广泛使用的裂解缓冲液。增溶剂是 NP - 40,它们可被其它洗涤剂的不同浓度替代。因为 NP - 40 非离子洗涤剂 ，此裂解缓冲液具有较弱的效果比 RIPA 缓冲液。这可以用于当蛋白功能保留具有最小的...
Mopso 68399-77-9 Recipe for New Liquid Phase Separation Technology in Capillary Electrophoresis US$32.10-42.30 / kg Mopso Buffer 68399-77-9 Storage Sealed in Dry Room Temperature US$32.10-42.30 / kg Mops with High Purity and Good Water Solubility Analysis o...