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The primers used were 5′-GGAATTCATGGAGACGCCGCCGCTGCCTCCCG-3′ and 5′-AGCGGCCGCCTAATGGTTTATCAATAACTGTGACC-CCTG-3′. The 1.5 kb cDNA was cloned into pGEX-5X-1. 2.2. Purification of recombinant GST fusion proteins An overnight culture (250 ml Luria-Bertani broth (LB), 100 μg/ml ...
GGAGCCACTGCCTTCATAGTCAA BCL6F GACTTCATGTACACATCTCGGC BCL6R CATCAGCATCCGGCTGTTGA ZBTB28F CTACGTCCGCGAGTTCACTC ZBTB28R CCCGGAAAATTGAATAGAAG β-actinF GTCTTCCCCTCCATCGTG β-actinR AGGGTGAGGATGCCTCTCTT MSP ZBTB16m3 CGGTGATATCGGAGTTCGTC ZBTB16m4 GCGTACGAAAATATACGAAACG ZBTB16u3 GTGGTGATA...
The primers used for polymerase chain reaction of PATZ1 (forward, 50-ACTTGGGCTTCCCTTTGG-30; reverse, 50-GC ACTGGATGCCACACTG-30; 229 bp), MnSOD (forward, 50-TTGGCCAAGGGAGAT GTTAC-30; reverse, 50-CTGATTTGGACAAGCAGCAA-30), catalase (forward, 50-AG GCCAGTCCTGACAAAATG-30; reverse, ...
Figs. 3A–F summarize the results of a study using a solid-phase based immunoblot assay to assess the relative levels of TGF-β3 and TNFα in SCCM, GCCM versus testes (Figs. 2A, C) by plotting the relative amount of corresponding cytokine in these media/lysates against the recombinant ...
(m-BTBD2T), the coding region of BTBD2 from the second ATG to almost the end of the 3'-UTR (445–2542 bp) was amplified with primers (Upper primer: 5'-ATGTTCAACGGGGGAATGGCCACAACATC-3', Lower primer: 5'-GGGGAGGACGCAGGTTCCAGGAGT-3') containing BamH I and Hind III cleavage ...
To generate the P-element encoding the GFP-CP190dBTB, we performed PCR using the full-length CP190 cDNA (LD02352, Research Genetics) as the template and the 5'-caccgagaacgttaatcgccag-3' and 5'-tagctcctccttcgccgc-3' as the primers. The amplified CP190dBTB fragment was inserted into ...
[eihpptpl1tpcuVViprapp1iiioiplasennStSo,aoen1nesgCCagstdts,2dsirscsassoas]sfaiti.newcbwcinnbssnvhrhdluudlsiseiaweeettrrfththmttmeefeefihhhoortlldyahyeeeeesarr, the power reference with the second power control scheme is represented by Pt∗otal (t) = Pinitial + [Pres(t − T1) × ...
=== D2X ===http://icculus.org/d2xUseful Links: === Nasm:http://nasm.sourceforge.netSDL:http://www.libsdl.orgPhysicsFS:http://icculus.org/physfs/Linux/Unix: === Required tools: * GCC 2.95 or higher * SDL 1.2 * PhysicsFS * NASM 0.98 (OPTIONAL but RECOMMENDED for x86) * A thread...
cattccagcgaggagagtgggtatgccagtgtggctggacagagcctccctgggcccatg gtggaccagagcccttcagtctccacttcatttggtctttcagccatgagtcccaccaag gctgcagtggacagtttgatgaccataggacagtctctcctgcagggaactcttcagcca cctgcagggcccgaggagccaactctggctgggggtgggcggcaccctggggtggctgag gtgaagacggagatgatgcaggtggatgaggtgcccagccaggacagccct...