Thermo Scientific Hin1I (BsaHI) restriction enzyme recognizes GR^CGYC sites and cuts best at 37°C in G buffer. SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also...
Thermo Scientific Hin1I (BsaHI) restriction enzyme recognizes GR^CGYC sites and cuts best at 37°C in G buffer. SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also...
但是,当进行抗体生物素化(Biotinylation)、荧光染料标记(Fluorescent dye labeling)、酶标记(Enzyme Labeling)、胶体金标记(Immunogold labeling)等实验时,BSA会与抗体形成竞争关系,大大降低抗体的偶联效率。此外,在进行抗体片段化(Antibody fragmentation)、共价固定(Covalent immobilization)等其它实验时,BSA的存在也是一种...
Thermo Scientific FastDigest Hin1I restriction enzyme recognizes GR^CGYC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.Isoschizomers: BsaHI, AcyI, BssNI, BstACI, Hsp92I. Thermo Scientific FastDigest Hin1I is one of an advanced line of fast restriction enz...
Ultrasensitive ELISA using enzyme-loaded nanospherical brushes as labels Authors:Qu Z, Xu H, Xu P, Chen K, Mu R, Fu J, Gu H. Journal:Anal Chem (2014): 9367 3-(10'-Phenothiazinyl)propionic acid is a potent primary enhancer of peroxidase-induced chemiluminescence and its application in se...
The interaction mechanism of four angiotensin-converting enzyme inhibitors (ACEI) with Bovine Serum Albumin (BSA) was studied by spectroscopy, the computer simulation technology is used to predict and explain the interaction mechanism. The former can understand and characterize the key parameters of the...
The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of ...
检测相思子毒素BSA-ELISA条件的优化1 * 罗胜军 王哲 高英杰 李小兵 刘国文 谢光洪 徐慧娟 马惠海 江涛 孙广瑞 (吉林大学畜牧兽医学院吉林 长春 130062 ) 摘要: 目的 应用生物素-链霉亲和素酶联免疫吸附法(BSA-ELISA)建 立相思子毒素(Abrin)测定法,并初步应用人工污染香肠及生理盐水 样品中相思子毒素回收率的检测...
Carnitine acetyltransferase (CRAT) is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. ...
Microbial xylanases have a promising biotechnological potential to be used in industries. In this study, regulation of xylanase production was examined in Bacillus cereus BSA1. Xylanase production was induced by xylan. The enzyme production further increased in the presence of xylose and arabinose in...