and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600085) at 1:500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour a...View ...
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Overview for how to clean and maintain CeramicSpeed Bearings used in Bottom Brackets and Wheel Hubs. It is always important to follow these instructions and apply the proper maintenance to your CeramicSpeed Bottom Brackets and Bearings in order to achieve the lowest friction and the best longevity....
1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis...
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10,000 units/ml。 37℃ 时活性 20%。 甲基化敏感性 对dam、dcm和哺乳动物 CpG 甲基化均不敏感。 注意事项 BsaWl 是 Betl 的完全同裂酶。 微信号 15221999938 联系我们 上海金畔生物科技有限公司 电话:021-50837765 手机:15221999938 网址: www.jinpanbio.com ...
The concentrations of metal acetate and sodium selenite used were always 1:1 (i.e., 1.25 mM in each case) and that of hydrazine was fixed at 0.78 M. The amount of BSA was changed systematically from 1−10 × 10−4 g/mL to determine its influence on the crystal growth of these ...
Chromatin Immunoprecipitation (ChIP) 1:10-1:500 Gel Super Shift Assays reported in scientific literature (PMID 11325839) Immunoblotting 1:100 - 1:2000 Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 25343232) Immunohistochemistry 1:100 Immunohistochemistry-Paraffin 1:100 Immunop...
Use in Knockdown Validated reported in scientific literature (PMID:32828953)In Western blot a band can be seen at approx. 29 Knockdown Validateda. Gel Super Shift Assays was reported in scientific literature. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point...
All the specific primers used in this study were designed via Primer 3.0 software and are listed in Supplemental Table 1. qRT‒PCR was conducted on a CFX96 Touch Real-Time PCR machine (Bio-Rad) with TransStart®Green qPCR SuperMix (TRANS), and three replicates were included in the anal...