Hapten sulfaguanidine (SG) was coupled with carrier protein bovine serum albumin (BSA) to form a full antigen SG-BSA by diazotization and glutaraldehyde me... ZY Zhu,CX Zhan,QZ Zhang - 《东北农业大学学报(英文版)》 被引量: 0发表: 2014年 Synthesis and Identification of SG-BSA and SG-...
CutSmart、重组酶、省时酶。 反应条件 CutSmart 缓冲液,60℃。 热失活:80℃ 20 分钟。 浓度 10,000 units/ml。 37℃ 时活性 20%。 甲基化敏感性 对dam、dcm和哺乳动物 CpG 甲基化均不敏感。 注意事项 BsaWl 是 Betl 的完全同裂酶。 微信号 ...
CutSmart、重组酶、省时酶。 反应条件 CutSmart 缓冲液,37℃。 热失活:80℃ 20 分钟。 浓度 10,000 units/ml。 甲基化敏感性 对dcm和哺乳动物 CpG 甲基化敏感。 注意事项 BsaHl 是 Ahall 的完全同裂酶。 在60℃ 温育活性提高两倍。 微信号
Check access to the full text by signing in through your organization. Access through your organization Section snippets Materials and methods All the syntheses of the complexes were performed under argon atmosphere. Solvents were purified by standard methods. All chemicals used were of reagent grad...
Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward Applications/Dilutions Dilutions Flow (Intracellular) Flow Cytometry Immunoblotting reported in scientific literature (PMID 28264879) ...
In order to investigate the biological properties of the prepared complexes, spectroscopic and biological studies were performed. Results of X-ray diffraction showed that1and2form two types of crystal structures in a given system: dinuclear and mono-nuclear complex. The preliminary study on the DNA...
Full size image Figure 3 Synthesis of bisbenzimidazole derivatives30and31. Full size image Under Suzuki-coupling conditionsviz., Pd(PPh3)4, K2CO3in CH3CN and water, intermediate4was reacted with dibromo imidazo[1,2-a]pyrazine5to yield 4-(6-bromoimidazo[1,2-a]pyrazin-8-yl)-N-cyclohexyl...
Cell fractionation of the liver measured the concentration of radioactivity that accumulated in the hepatocytes, endothelial cells, and Kupffer cells after the injection of 111In-LDTPA galactose BSA. Access through your organization Check access to the full text by signing in through your organization...
Full scan mode was set as the mass detection mode from 100 m/z to 1000 m/z. 4. Conclusions In this study, BSA bound ligands in Radix astragali were screened out and identified by BSA-MN coupled with HPLC-MS. The BSA-MN was prepared and characterized by TEM, XRD, FT-IR and VSM....
MiSeq (Illumina) using 250-bp paired-end chemistry (New Zealand Genomics Limited, Palmerston North, New Zealand). The resulting 2.6 million sequences (991 million base pairs) were trimmed of low-quality results using a 0.01 quality cut-off in DynamicTrim in SolexaQA (Cox et al.,2010), ...