BrdU Incorporation Assay to Analyze the Entry into S Phase Chapter © 2022 Diagnosis and Pathological Analysis of Patient Cancers by Detection of Proliferating Cells in Gelfoam® Histoculture Chapter © 2018 Impact of antigen retrieval protocols on the immunohistochemical detection of epigenetic ...
Incorporation of bromodeoxyuridine (BrdU) during DNA replication is frequently used for cell cycle analysis. The flow cytometric BrdU/Hoechst quenching technique is conducive to high-resolution assessment of cell cycle kinetics, but requires continuous BrdU treatment, which may have cytostatic or cytotoxic...
De Fraissinette A., Staquet, M. J., Dezutter-Dambuyant, C., Schmitt, D.&Thivolet, J.(1988) Langerhans cells in S-phase in normal skin detected by simultaneous analysis of cell surface antigen and BrdU incorporation.J. Invest. Dermatol.91, 603–5. PubMedGoogle Scholar De Greef, G....
BrdU incorporation in cyst cells (1.4% [95% CI: 1.0 to 2.1]) was greater than in OSE (0.7% [95% CI: 0.4 to 1.2]) and very few BrdU-labeled cells were observed in rete ovarii at any age. Incessant ovulation significantly increased BrdU incorporation in OSE of older animals. ...
The assay was robust up to 30,000 cells per well. BrdU dot plots of cell cycle phases showed an excellent separation of cell populations, and DNA histograms showed a low coefficient of variation. Thermal denaturation was suitable for 96-well plates to detect BrdU incorporation with a good ...
BrdU incorporation in cyst cells (1.4% [95% CI: 1.0 to 2.1]) was greater than in OSE (0.7% [95% CI: 0.4 to 1.2]) and very few BrdU-labeled cells were observed in rete ovarii at any age. Incessant ovulation significantly increased BrdU incorporation in OSE of older animals. ...
A rapid in vitro assay system using anti-bromodeoxyuridine for drug susceptibility of Plasmodium falciparumCyclingmuscle activityLombard's paradoxkinesiological EMGDrug effects were monitored by the measurement of bromodeoxyuridine (BrdU) incorporation into parasite deoxyribonucleic acid (DNA). Cells are pulse...
A more recent non-isotopic variation of the assay utilizes bromodeoxyuridine (BrdU) incorporation in vivo . To further improve the utility of this assay, we developed an ex vivo BrdU labeling procedure eliminating the need for in vivo injections. The results of this assay correctly identified a ...
BrdU incorporation into nuclei was detected immunohistochemically, allowing estimation of a proliferation index for different organs and cell types. Bath exposure to BrdU will be useful in study of the response of organisms to chemicals such as epigenetic carcinogens that alter rates of cell ...
A variety of clinical reports have described the application of the bromodeoxyuridine labeling index as an adjunct to conventional pathological examination of CNS tumors. This index has proven useful in predicting the clinical outcome associated with many such tumors. Furthermore, because of its efficacy...