In this paper, we propose a novel microscopy image translation method for transforming a bright-field microscopy image into three different fluorescence images to observe the apoptosis, nuclei, and cytoplasm of cells, which visualize dead cells, nuclei of cells, and cytoplasm of cells, respectively....
Korzynska A, Iwanowski M: Multistage morphological segmentation of bright-field and fluorescent microscopy images. Opt-Electron Rev 2012, 20 (2) : 87–99.Korzynska A, Iwanowski M: Multistage morphological segmentation of bright-field and fluorescent microscopy images. Opt-Electron Rev 2012, 20(...
Remarkably, transmission OPT, which provides bright-field contrast, is used to correct absorption artifacts in Light Sheet Fluorescence Microscopy (LSFM)20,21 and for multimodal reconstruction of the whole specimen’s anatomy22. The idea behind OPT is to acquire images (or projections) of the sampl...
Once candidate objects of interest are identified, further analysis is conducted automatically to process and collect data from samples having different staining agents.Torre-bueno, Jose De LaMcbride, James
A hamster dorsal skin fold model in combination with brightfield/fluorescence intravital microscopy was used to examine the effect of laser pulse duration and blood flow velocity on the size of the thermal coagulum, its attachment , and laser-mediated vasomotion. The size of the coagulum and the...
摘要: The tiniest structures in cells can be seen only using sophisticated instrumentation and informatics, but what biologists really need are improved fluorescent probes.关键词: Fluorescent Dyes Microscopy, Fluorescence Cell Survival Molecular Imaging Light Fluorescence ...
Imaging of the liquid-liquid interface under electrochemical instability using confocal fluorescence microscopy. The onset of the electrochemical instability has been imaged at the 1,2-dichloroethane (DCE)|water (W) interface modified with a fluorescent phospholipid u... Y Kitazumi,T Kakiuchi - 《Lan...
(TTM) based on a field-programmable gate array that can tag in parallel multiple single-photon events, with 30 ps precision, and multiple synchronisation events, with 4 ns precision. We use the TTM to demonstrate live-cell super-resolved fluorescence lifetime image scanning microscopy and ...
The BUC5F series cameras can be widely used in bright field, dark field, fluorescent light environment and normal microscope image capture and analysis with higher frame rate. Feature The basic characteristic of BUC5F series cameras are as follows: ...
The limitations of classical bright-field microscopy include low contrast for weakly diffracting, absorbing, and autofluorescent samples and low resolution due to the blurry appearance of out-of-focus material. However, bright-field transmission microscopy can allow the observation of unlabeled living cel...