In this chapter, a basic theory of annular bright field (ABF) STEM is described using the so-called "s-state model" based on the channeling theory for dynamical electron diffraction. The effectiveness of this method for observing complex structures and dopant atoms in crystals was already ...
High angle annular dark field(HAADF) and annular bright field(ABF) imaging of the aberration-corrected STEM are widely used due to their high-resolution capabilities and easily interpretable image contrasts. However, HAADF mode of the STEM is still limited in detecting light elements due to the ...
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It has generally been assumed that a transmission electron microscope is used and no consideration has been given to a difficulty which arises if the microscope is of dedicated scanning transmission type (STEM). The problem is that the parallel detector takes the place of the bright-field ...
A Monte Carlo electron-trajectory calculation has been implemented to assess the optimal detector configuration for scanning transmission electron microscopy (STEM) tomography of thick biological sections. By modeling specimens containing 2 and 3 at% osmium in a carbon matrix, it was found that for 1...
Image acquisition Widefield and epifluorescence images were acquired using a Zeiss Axiocam MRc camera coupled to a Leica M165FC dissecting scope or a Zeiss A1 Axioscope. Laser scanning confocal data were acquired using a Zeiss LSM510 META using a PlanApo 20×/NA0.75 objective. Fluorophores were...
The intestine is an organ which self-renews its lining every few days, much owing to the presence of intestinal stem cells (ISC) in the crypts17. Fibroblasts, along with other cells, encompass a supporting niche for leucine-rich repeat-containing G-protein 5 (Lgr5)+ISC18,19(Fig.1a) and...
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aElectron density (green-yellow) and longitudinal fieldEx(blue-red) att = − 16T0, in which `A', `B' and `C' mark three different electron nanobunches in space, but can also be used to phenomenologically represent the compression, injection and acceleration stages of the same elec...
Reactive oxygen species (ROS) constitute a group of highly reactive molecules that have evolved as regulators of important signaling pathways. It is now well accepted that moderate levels of ROS are required for several cellular functions, including gene