The present invention relates to a process in vitro for the detection of chromosomal abnormalities in a mammal, comprising the in situ hybridization of chromosomes of a preparation of chromosomes metaphase with sets of a plurality of nucleic acids, each assembly which hybridize, over a length of ...
Metaphase spreads were obtained after a 1 h 15 min colcemid/karyomax (Gibco) treatment followed by incubation in a hypotonic solution. Cells were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Vector). BAC DNA probes were labeled using Alexa flour dyes (488, green and 594, red)...