正是在这样的背景下,2024年6月26日,来自Arc研究所的Patrick Hsu团队在Nature期刊同期发表了两篇研究论文,描述了一种受桥 RNA(Bridge RNA)引导的重组酶的特性,其能够在特定基因组位点插入、倒位或删除长片段 DNA 序列,从而使这些重组酶能够重编程以实现新的基因组编辑能力。桥RNA的发现不仅为理解基因组重排机制提供了新的
This bridge RNA contains two internal loops encoding nucleotide stretches that base-pair with the target DNA and the donor DNA, which is the IS110 element itself. We demonstrate that the target-binding and donor-binding loops can be independently reprogrammed to direct sequence-specific recombination...
Here we report that IS110 insertion sequences, a family of minimal and autonomous mobile genetic elements, express a structured non-coding RNA that binds specifically to their encoded recombinase. This bridge RNA contains two internal loops encoding nucleotide stretches that base-pair with the target...
BridgeRNA2024 This code repository accompanies the paper "Bridge RNAs direct programmable recombination of target and donor DNA" by Durrant & Perry et al, 2024. It includes snakemake pipelines and code for various key analyses described in the paper. Contents There are 5 separate pipelines in this...
基因编辑CRISPR引发了生物学的一场革命,现在有不少技术进入临床或临床研究,前景非常广阔;加州弧光研究所(Arc Institute)的科学家在细菌中发现了更为强大的编辑系统——'桥式编辑“或”桥连编辑“,因为能把2条DNA链”桥连“在一起,可做DNA大片段的重排、缺失、颠换和插入,非编码RNA做”桥“,非常精准,有可能解决...
内容摘要Bridge RNA 是新出现的一种对活细胞做基因编辑的方法。它可以几乎无缝、无疤地基因组做大片段基因的插入,而且在插入的靶标位点选择、供体序列选择上有很大的灵活性。同时,Bridge RNA 的脱靶编辑概率较低。另外,Bridge RNA 还可以做大基因片段的删除、和颠倒方向
RNA Biol. 6, 138-140.Non-coding RNA:a bridge betweensmall RNA and DNA. Kurth HM,Mochizuki K. RNA Biol . 2009Kurth HM, Mochizuki K (2009). Non-coding RNA: a bridge between small RNA and DNA. RNA Biol 6, 138-140.Kurth HM, Mochizuki K: Non-coding RNA: a bridge between small ...
由佛罗里达州斯克里普斯研究所的Wanqian Li发表的名为“Decreasing the intrinsically disordered protein α-synuclein levels by targeting its structured mRNA with a ribonuclease-targeting chimera”的论文中,研究人员从ChemBridge化合物库中筛选出能够结合RNA的化合物,并从这些化合物中发现了能够改善帕金森综合征的新型...
By using those reliable scATAC-seq cells to bridge RNA and ATAC omics, scBridge achieves better integration results (63.62% label transfer accuracy) in the second iteration. As the training proceeds, more scATAC-seq cells are selected as reliable by scBridge, and the label transfer accuracy ...
Given the apparent limitation of double-stranded RNA (dsRNA) genomes to about 30 kb, together with the complexity of DNA synthesis, it appears difficult for a dsRNA genome to encode all the information required before the transition from an RNA to a DNA genome. Ribonucleotide reductase itself, ...