Data analysis for margin evaluation The optical probe records 12 Raman spectra corresponding to three rings/quadrant for four quadrants at each point that was averaged for each spot over a sampling depth of 2 mm and a spot size of 7 mm diameter in this study. Neon-argon lamp, naphthalene, ...
The percentage positive cells in the lower right (APC positive/PI negative: early apoptotic cells) and upper right (APC positive/PI positive: late apoptotic cells) quadrants were summed to calculate the total number of apoptotic cells. Conflict of Interest The authors declare no conflict of ...
which not only promoted MHC-I expression but also caused immunogenic cell death of breast cancer. Finally, the scRNA-seq confirmed the negative correlation of PIP5K1A et al. with TILs in breast cancer patients. Collectively, we identified multiple gene targets for an increase in MHC-I expression...
For ALDH1 detection, cells were subjected to the AldeRed ALDH Detection Assay (Millipore, Burlington, MA, USA, #SCR150) with diethylaminobenzaldehyde (DEAB)-treated cells as a control to inhibit ALDH activity. To determine appropriate gating for CD24 and CD44 quadrants, stained parental MCF7 ...