3. Li, X., Darzynkiewicz, Z. 1995. Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation.Cell Prolif. 28: 571. D) F-Actin/PI protocol D.2.1 Materials PFA (A2), PBS, Triton X-100 (A2), sodium borohydride, FITC-phalloidin (A2), PI (A2). D.2.2....
碘化丙啶(PI)是一种不能透过活细胞膜的核酸染料,仅在凋亡晚期细胞膜通透性增加后入核,嵌入DNA后可激发出红色荧光。以图8流式结果展示说明[6],落在Q4区(Annexin V-/PI-)的属于正常细胞,落在Q3区(Annexin V+/PI-)的细胞处于凋亡早期,而位于Q2区(Annexin V+/PI+)的则是凋亡晚期的细胞,落在Q1区(Annexin ...
DNA content is revealed by red fluorescence from PI. In order to have more details, see the Chapters related to TUNEL technique. D) F-Actin/Propidium Iodide The analysis of apoptotic cells and estimation of their cell cycle specificity is also possible using a recent method. This is based ...
dna contentis revealed by red fluorescence from pi. in order to have more details, seethe chapters related to tunel technique.d) f-actin/propidium iodidethe analysis of apoptotic cells and estimation of their cell cyclespecificity is also p 20、ossible using a recent method. this is based on...
estimationoftheir cell cycle specificityisalso possibleusingarecentmethod、This isbased onidentification ofapoptoticcells which havemodifiedtheir cytoskleton andtheir DNAcontent.Inspecific,paraformaldehyde (PFA) fixation followed bystainingof F-actinwith fluorescein-conjugated phalloidin and ofDNA withPI,are ...
An annexinV/propidium iodide (PI) assay was used to identify potential induction of apoptosis by BrdU. Proliferative activity in BT474, SK-BR-3, and RT4 cultures was reduced in different cell cycle phases due to continuous treS.Institute...
A) BrdU/PI PROTOCOL A。2、1 Materials BrdU (A2), washing buffer (A1), HCl 4 M, Borax buffer (A1), anti—BrdU antibody (A2), goat-anti—mouse-FITC antibody (A2), PI buffer (A1)。 A.2。2、 Methodology 1。 Cells (1x106 /mL) are incubated with BrdU 10 m M at final concentra...
A) Bromodeoxyuridine/Propidium Iodide The classical method for the analysis of cell cycle distribution is the flow cytometric measurement of DNA content which can simultaneously determine the incorporation of Bromodeoxyuridine (BrdU). The procedure requires that DNA is partially denatured to expose ...
细胞内的DNA含量随细胞周期进程发生周期性变化,如G0/G1期的DNA含量为2C,而G2期的DNA含量是4C。利用PI标记的方法,通过流式细胞仪对细胞内DNA的相对含量进行测定,可分析细胞周期各时相的百分比。 实验步骤: 一、取对数生长期细胞,倒去培养液,胰酶适度消化细胞,用培养液吹打,800 rpm,离心15 min去上清。
DNA content is revealed by red fluorescence from PI. In order to have more details, see the Chapters related to TUNEL technique. D) F-Actin/Propidium Iodide The analysis of apoptotic cells and estimation of their cell cycle specificity is also possible using a recent method. This is based ...