图10. 凋亡细胞的DNA电泳结果图 (A为marker,B为正常细胞,C-E为凋亡细胞) (2)原位末端缺口标记法(TUNEL法):断裂的DNA会产生大量的粘性3’-OH末端。TUNEL法的原理是通过用重组末端脱氧核苷酸转移酶(rTdT酶)在断裂DNA的3’-OH末端催化掺入带有荧光素标记的dUTP,可采用荧光显微镜或流式细胞仪进行定量分析。如图11...
图10. 凋亡细胞的DNA电泳结果图 (A为marker,B为正常细胞,C-E为凋亡细胞) (2)原位末端缺口标记法(TUNEL法):断裂的DNA会产生大量的粘性3’-OH末端。TUNEL法的原理是通过用重组末端脱氧核苷酸转移酶(rTdT酶)在断裂DNA的3’-OH末端催化掺入带有荧光素标记的dUTP,可采用荧光显微镜或流式细胞仪进行定量分析。如图11...
注意:为了便于后面分析结果,我们一般会选用已确定分子量大小而且纯度高的抗体作为marker与一抗同时孵育。 5-溴脱氧尿嘧啶核苷抗体BrdU免疫标记,免疫组化实验操作步骤(以石蜡切片为例) 仪器、设备: 移液枪、免疫组化笔、水浴锅、计时器、孵育湿盒、切片架、盖玻片、光学显微镜、洗瓶等。
5-溴脱氧尿嘧啶核苷(增殖标志物)抗体(BrdU(Proliferation Marker)) 价格￥1980 起批量≥1支 最小起订1支 供货总量50支 发货地址浙江省温州市 建议售价￥1980/支 更新日期2025年03月15日 产品规格100ul 即时洽谈立即询价查看联系方式 收藏产品发送留言
The Ki-67 protein (MKI67) is a cellular marker for cell proliferation (Figure 3). Ki67 is present during all active phases of the cell cycle (G1, S, G2, and M) but is absent in resting cells (G0). The nuclear expression of Ki67 can be evaluated to assess tumor proliferation by ...
Bromodeoxyuridine, Bromodeoxyuridine,5-Bromo-2′-deoxyuridine; 5-BrdU, Proliferation Marker; 5-Bromo-2-deoxyUridine Immunogen KLH conjugated Synthetic peptide corresponding to Mouse BrdU Isotype IgG1,κ Purity Affinity purification Subcellular location Nucleus Applications IHC/IF All species 1: 100-1: 500...
BrdU,英文全称5-bromo-2'-deoxyuridine,中文全称5-溴-2’-脱氧尿苷,是一种合成的胸苷溴化类似物,在S期可替代胸苷选择性插入细胞DNA。BrdU通常和广泛用于测定DNA合成和标记分裂细胞,最后用来研究诱导细胞增殖的信号通路和其他生理过程。BrdU通过体外细胞培养或体内注射的方式进行探针加载,然后用抗BrdU的抗体进行特异性检测...
they stain different groups of cells. BrdU labels cells during the S phase, whereas Ki67 labels cells in the G1, S, G2, and M phases (only Go cells should be negative for Ki67). However, using only one marker may yield only partial information, while using both will ensure and enhance...
Using CHIP-seq analysis targeting the DNA double-strand damage marker γ-H2AX, a correlation was found between its level and the ATAC-seq for Gata4 and Sox17 (Fig. 3H). This suggests that the activation of the Gata4 and Sox17 genes is mediated through the DNA damage repair pathway. ...
These unexpected findings raised the possibility that BrdU might have interfered with the mechanism of neurogenesis, as follows: BrdU, the first marker used, incorporated into dividing VZ mother cells; these gave rise to BrdU+ neurons which migrated to their final destination in the brain, where ...