Analysis of Brd4 conditional knockout (KO) mice showed that BRD4 is required for hematopoietic stem cell expansion and progenitor development. Nevertheless, BRD4 played limited roles in macrophage development and inflammatory response to LPS. ChIP﹕eq analysis showed that despite its limited importance,...
Brd4 is a prominent drug target for cancers but its role in normal cell differentiation and tissue development is largely unexplored. In this study, we use adipogenesis and myogenesis as model systems to explore the role of Brd4 in differentiation and development. Using tissue-specificBrd4KO mice,...
JQ1 can block the TGF-β1/Smad signaling pathway, attenuate the binding of BRD4 to theCol4a3promoter, and inhibit the activation and nuclear translocation of SOX9 in the kidneys of NTS-treated mice [249]. Similarly, Sandra Rayego Mateos et al. showed that JQ1 inhibited the expression of pr...
Furthermore, mice with a heterozygous deletion of BRD4 developed delayed heart failure, suggesting that even a reduced level of BRD4 protein is extremely harmful to heart function [98]. Collectively, these studies suggest that BRD4 is crucial for maintaining cardiac mitochondrial function and normal ...
o and p A2780-WT or TGF-β knockout (TGF-β KO) cells were injected into the subcutaneous of nude mice (n = 5 mice). The in vivo tumor size was monitored and tumors were dissected. ** P < 0.01. q CCK8 assays were performed in A2780 cells transfected with sh-PRMT1 and cells ...
o and p A2780-WT or TGF-β knockout (TGF-β KO) cells were injected into the subcutaneous of nude mice (n = 5 mice). The in vivo tumor size was monitored and tumors were dissected. ** P < 0.01. q CCK8 assays were performed in A2780 cells transfected with sh-PRMT1 and cells ...
S7J). The increased ROS generation and LC3 expression, and decreased expression of BRD4 in Cur-treated Apoe–/– mice were also reversed in macrophage-specific Tfeb KO mice (Fig. 7K and L, Fig. S7L). We also confirmed that the decreased P300 activity in the presence of Cur was also ...
(E) protein, a transmembrane protein involved in CoV virion assembly and pathogenesis of the related virus, SARS-CoV.1,4-6 In mice, knock-down of Brd4 is embryonic lethal.2 Chromosomal translocations leading to the fusion of BRD4 with the nuclear protein in testis (NUT) gene and resulting ...
However, Brd4 KO mice that survived the acute phase showed significantly higher ejection fraction than control 10 weeks after TAC. Despite the basal hypertrophy, Brd4 KO mice showed no further pathologic remodelling in response to PO and had significantly lower LV weights and diameters. CONCLUSIONS....
Furthermore, inhibition of Brd4 function alleviates transcriptional disruptions observed in Fmr1 KO neurons and reverses behavioral phenotypes observed in KO mice. This work demonstrates that loss of FMRP results in widespread epigenetic misregulation and that targeting transcription can alleviate deficits in...