CX-5461 induced more apoptotic cell death inBRCA2knockout cells relative to WT. However,BRCA2+/+andBRCA2−/−isogenic cells in HCT116 appeared equally sensitive to actinomycin (an inhibitor for both RNA polymerase I and II) and cycloheximide (an inhibitor for protein translation...
has a critical role in mitosis progression.21 To test the hypothesis that DNA-PKcs is involved in Chk2 activation during mitosis, human colon cancer HCT116 cells and isogenic DNA-PKcs knockout cells (DNA- PKcs À / À ) were mitotically synchronized or were treated with ionizing radiation...
This clonogenic screen identified CX-5461, a previously described RNA pol I inhibitor15,17 to be highly toxic to BRCA2 knockout HCT116 cells as compared with isogenic BRCA2 WT cells (Fig. 1a). We extended the quantification of this observation by using a WST-1 metabolic/ cell viability ...