kefiranofaciens, and the shotgun metagenomic analysis facilitated this investigation. Previous work reported the isolation of these bacteria from kefir and named the genome L. kefiranofaciens ZW3. However, there are some differences compared to our results. For instance, the genome of L. kefir...
“Shotgun” molecular cloning of putative antimicrobial peptide precursor-encoding cDNAs Using the molecular cloning strategy described, two different full-length cDNAs were consistently cloned from the Tityus serrulatus venom-derived cDNA library, each encoding a single copy of a putative antimicrobial pe...
not even seven days after we first dropped the single, we found out he got shot in Vegas. And we pulled the record from the radio — purposefully.
The whole genome shotgun projects have been deposited at DDBJ/EMBL/GenBank under the accessions CP022883.1 (Chromosome I) and CP022884.1 (Chromosome II). Conflict of interest statement The authors declare no competing financial interests. Authors' contributions BAM, MBH, and MKH designed and perform...
Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system using the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA), as recommended by the manufacturer. Quality filtering was done with version 0.36 of ...
core mycobiome. The case study of the same adult tree (previously studied using culture-dependent approach) analyzed by amplicon, shotgun metagenomics, and small RNA transcriptomics revealed a high relative abundance of insect parasite-pathogens, anaerobic fungi and a high expression ofTrichoderma(a fu...
Fixed an issue that caused the Hillbilly's shotgun to experience visual desynchronization after crashing into a wall. Fixed an issue that caused Survivors not to be sacrificed to the Entity when hiding in a locker at the end of the End Game Collapse timer. ...
Warren RL, Choe G, Freeman DJ, Castellarin M, Munro S, Moore R, Holt RA. Derivation of HLA types from shotgun sequence datasets. Genome Med. 2012;4:95. Warren RL, Holt RA. Targeted assembly of short sequence reads. PLoS ONE. 2011;6: e19816. ...
The advantage of the pHMM usage to screen type I PKS in metagenomic shotgun data and the possible recovery of false positives was discussed on a study performed by Foerstner et al. [39]. Table 1 summarizes the results of these analyses. From the 84 type I KS (confirmed by Blast and/...