The aim of this thesis is to describe a minimally supervised method to segment sketches coinciding with slices of the brainstem featuring labeled contours. This thesis also describes a deformable contour model approach, emphasizing a 1-simplex framework, to reconstruct a 3D volume from 2D slices....
Animals have evolved specialized neural circuits to defend themselves from pain- and injury-causing stimuli. Using a combination of optical, behavioral and genetic approaches in the larval zebrafish, we describe a novel role for hypothalamic oxytocin (OX
respectively. We examined how acute novelty stress influences behaviors in EPM and OFT (Supplementary Fig.1a). 2-h novelty stress significantly decreased time mice spent on the open arm of the EPM (Fig.1e, f),
Fig. 1: Acute novelty stress decreases food intake, induces anxiety and activates NPYDRN/vlPAGneurons. aAcute novelty stress paradigm. Group-housed mice were individually transferred into new cages without padding to induce acute novelty stress. 4-h (b), 4–24-h (c) and 24-h (c) food i...
data of the observed neuronal connections in the form of text or tables. These ordinal measures represent an estimate of connection densities. Level 3 is based on either stereological quantification of perikarya labeled by tract tracing or densitometric quantification of labeled axonal terminals. Semiqu...
labeled sequentially from I to VII. Typically, only waves I, III, and V are used clinically(eSlide 50.6). ABR testing is used for differential diagnosis (e.g., detection of the effects of an acoustic tumor),intraoperative monitoringduring tumor removal surgery,auditory thresholdestimation, and ...
Moreover, biotinylated dextran amine injection in the basal turn of scala media of a normal cochlea labeled cochlear nerve fibers projecting to the high-frequency regions of the cochlear nuclei and to the flocculus, but not to more central auditory nuclei. This is the first detailed account of...
These ChAT-LI fibers appear to branch extensively and cover all parts of the pontine nuclei. Following injections of rhodamine-B-isothiocyanate (RITC) in the thalamus and Fluoro-Gold in the pontine nuclei and surrounding regions in the same animal, all retrogradely labeled cells in the ...
To find the neurotransmitter used by these various TA premotor neuron pools, we repeated the ΔG-rabies tracing in transgenic neonatal mice where glutamatergic or GABAergic neurons were labeled. TA premotor neurons within the rv-iRF were Vglut2+, those in other respiratory brainstem centers ...
a,bCTb injection into the RVLM unilaterally. TH tyrosine hydroxylase; IO inferior olive nucleus; LPGi lateral paragigantocellular nucleus; NA nucleus anbiguus. Scale bar: 1 mm.cDrawing of the distribution of CTb-labeled cells [combined from 8 rats (6 males, 2 females)] and CTb-positive...