2e–g). Long-term EGFP-cloneID expression was maintained in all major cell types of the juvenile mouse brain, and labeled cells were found in various regions (Fig. 1b,c). In conclusion, we present a highly diverse lentivirus library suitable for heritable and brain-wide labeling of ...
We next carried out direct experimental verification of the neuronal cell type assignment by the lipidome assessment of fluorescently labeled pyramidal neurons and unlabeled cells isolated using cell sorting from the brain of Thy1-ChR2-YFP45 transgenic mice (Fig. 5g; Supplementary Fig. 10). The ...
The estimated smooth function can be immediately visualized with the plot() function. The output is not shown, but see the curve labeled mod1a in Fig. 12 (left). We can check that the number of splines is sufficiently high with k.check(), which implements a permutation algorithm described...
The majority of the labeled neurons were seen in the forebrain, with particularly large numbers in the infralimbic cortex, lateral septal nucleus, and preoptic region. The anterograde tracing experiments supported the general results of the retrograde tracing experiments. However, we did not observe ...
The ADS comprises two main stages: the first acquires and transforms features in parallel that highlight the object, while in the second stage, all integrated features serve the goal of object detection. 3.3.1. Acquisition and Transformation of Features In this stage, different parts of the ...
The SMA receives an input of music note information, and the PMC encodes the information with 16 groups of population-encoding neurons for action selection. The cerebellum’s GC, PC, and DCN parts form a residual connection and receive the output of the movement population neurons. The DCN ...
We found no evidence of specific 'labeled-lines' among the odor-responsive interneurons, as none responded exclusively to one plant odor or pure odorant; most olfactory interneurons were broadly tuned in their response spectra. This finding is consistent with an 'across-fiber' pattern of odor ...
Together with former UC Berkeley professor John Ngai, Bateup and UC Berkeley colleague Dirk Hockemeyer have already used CRISPR-Cas9 to create mice in which a specific cell type is labeled with a fluorescent marker, allowing them to track the connections these cells make throughout the brain. Fo...
The MBN and HDB were injected iontophoretically with the anterograde tracer Phaseolus vulgaris leucoagglutinin (PHA-L). After immunocytochemical visualization of labeled efferents, the distribution of projections over the cortical mantle, olfactory regions and amygdala were studied by light microscopy. ...
1b; Supplementary Data 1), consistent with short-term loss of SST interneurons reported in previous studies17,18. Fig. 1: SST interneuron loss after focal TBI. a Coronal section 8 wks after TBI labeled for SST-GFP (green) and DAPI (magenta). h, hilus; gcl, granule cell layer; ml, ...