Fig. 2: Atlas-level hierarchical cell type annotation. a, Schematic diagram illustrating the scAnnot tool (created withBioRender.com). This hierarchical classification model, based on scANVI, categorizes cells into cell types. The first level of classification groups cells into broad cell types, w...
First, we calculated the total number of cells at the time point of injection. If Nt1 is the number of labeled cells at E11.5, Δt is the time difference in days and f is the frequency of cell divisions per day, then the number of transduced cells Nt0 is: ...
We found that average controllability of the cingulo-insular system [labeled Salience/Ventral Attention A in the Yeo et al. 2011 atlas (30); nodes included in this functional system are listed in Table S2 in Supplement 1, and associations between these nodes and neurosynth meta-analysis maps ...
The insert shows surface rendering of a dendritic spine structure on a GFP+ neuron with the pre-synaptic terminal labeled by SV2 staining in red. Scale bars, 50 μm. Shown in (D, left) are sample recording traces of sEPSCs and pharmacological blockade by DNQX. Identified sEPSC events ...
[Abbreviations: NPCs: neural progenitor cells; Cort: cortical; ESCs: embryonic stem cells; DA: dopaminergic]. We checked for relative agreement between training and testing data composition prior to applying PCBrainAge to test data. We previously showed that it is possible to use PCs from one ...
(BV2_NC). A total of 2 × 107cells for each TMT labeling were harvested and lysed, and then mixed with protein lysates in a 1:1 ratio. After trypsin digestion, tryptic peptides were labeled with their respective TMT reagent. After labeling, the sample was fractionated into fractions ...
Human cerebral organoids undergo vascularization and maturation in the mouse brain. Differentiation of human pluripotent stem cells to small brain-like structures known as brain organoids offers an unprecedented opportunity to model human brain developme
Schematic diagram of the protective effects of FMT administration on the rotenone-induced PD mouse model through the microbiota-gut-brain axis. Briefly, FMT treatment restores the gut microbiota dysbiosis induced by rotenone, which decreases the generation of pathogenic LPS in the gut and inhibits the...
Hepatocyte-like cells differentiated from human induced pluripotent stem cells: relevance to cellular therapies. Stem Cell Res. 9, 196–207 (2012). Article CAS PubMed PubMed Central Google Scholar Xu, X. et al. Mitochondrial regulation in pluripotent stem cells. Cell Metab. 18, 325–332 (...
To assess the effect of MSC-Exos on BV2 senescence, we first confirmed whether MSC-Exos could be incorporated into BV2 cells. BV2 cells were labeled with DAPI (blue) and exosomes with DiO (green). The fluorescence results showed that the primary localization of green fluorescent dye (DiO)...