at 20 mg/kg body weight into L4-L6 vertebral spine for five consecutive days. DMSO was injected in parallel as Vehicle. Sodium-dependent vitamin C transporter 2 (Svct2) KO mouse embryos (C57BL/6 background) at embryonic day 14.5 were kindly provided by Dr. Fiona E. Harrison and have ...
Midbrain dopamine (mDA) neurons participate in a wide range of brain functions through an intricate regulation of DA biosynthesis. The epigenetic factors and mechanisms in this process are not well understood. Here we report that histone demethylase JMJD3 is a critical regulator for DA biosynthesis ...
Motile cilia defects impair cerebrospinal fluid (CSF) flow and can cause brain and spine disorders. The development of ciliated cells, their impact on CSF flow, and their function in brain and axial morphogenesis are not fully understood. We have characterized motile ciliated cells within the zebra...
we sought to further confirm this observation using an enzyme that cleaves only high-mannose and hybrid structures, known as endoglycosidase H (Endo H). The intensity of individual N-glycans isolated from the cortex using PNGase F (Fig.3A) was compared to those isolated by Endo H (...
A single axon was labeled and visualized without any breaks, making its trajectory easily traceable. In addition, the dendritic spines in the cerebral cortex of the same strain were clearly observed by two-photon microscopy (Figure S5B). Furthermore, proteins in the spine were also preserved in...
We demonstrate here that after brain injury, reactive glial cells including both astrocytes and NG2 cells can be reprogrammed into functional neurons in the adult mouse cortex when infected with retrovirus encoding a single transcription factor, NeuroD1. Electrophysiological recordings revealed both sponta...
Neurons within brain slices showed stable dendritic spine density and mature spine morphologies in the first weeks in culture, and they generated detectable activity in multi-electrode arrays. We infected cultured brain slices using patient-matched CD4+ T-cells or monocyte-derived macrophages (MDMs) ...
brain sciences Review Localization of Free and Bound Metal Species through X-Ray Synchrotron Fluorescence Microscopy in the Rodent Brain and Their Relation to Behavior Caroline L. C. Neely 1,* , Stephen L. P. Lippi 2 , Antonio Lanzirotti 3 and Jane M. Flinn 1 1 Department of Psychology, ...
medical sciences Review Disparities in Brain Cancer in the United States: A Literature Review of Gliomas Dharam Persaud-Sharma 1,*, Joseph Burns 1, Jeran Trangle 1 and Sabyasachi Moulik 2 1 Florida International University, Herbert Wertheim College of Medicine, Miami, FL 33199, USA; jburn052@...
Linker and binding affinity optimizations of GluSnFR gave rise to an enhanced probe, Super GluSnFR (44% ∆F/F), that was successfully used to quantitatively image synaptic glutamate spillover and reuptake in cultured hippocampal neurons with centisecond temporal and dendritic spine-sized spatial ...