The primers are described in Table S4. The constructed bait vector and BrPSY1 were co−transformed into yeast strain NMY51. All strains were grown in different media: (1) SD medium lacking Trp and Leu; (2) QDO (SD medium lacking Trp, Leu, His, and Ade); and (3) QDO + 3AT...
To determine whether GmBRI1b contains introns, we designed primers covering the initiation codon and stop codon and amplified the genomic DNA. After sequencing, we found that GmBRI1b also lacks introns. This indicated that the structure of the BR receptor genes has been highly conserved between...
napus genome, we performed a PCR analysis of the T0 and T1 generations using Cas9-888 and U6-26p primers to confirm the pos- itive transgenic plants. The Cas9 vector with T-DNA was not detected in 20 of 108 (18.51%) T0 plants and 35/145 (24.1%) T1 plants originating from 17 ...