建立索引。bowtie2-build是bowtie2建立索引的常用的指令, -f指定要索引文件后,再给予索引的名称。参考基因组通过bowtie2-build指令在Index文件中的索引文件的前缀,此前缀不包含索引文件的.1.bt2/.rev.1.bt2/etc.bowtie2等。
bowtie2-build Genome.fasta index genome.fasta :自己本身下载的fa文件 index:自己为之后建立的索引起的名字 回车 建库需要一段时间 之后会生成六个文件即可
bowtie2-build的使用 1.build index database use bowtie2 bowtie2-build -f genome.fasta genome -f 为参考基因组genome.fasta Genome为文件索引
* Add meta to bowtie build * Swap fastq ngscheckmate tests * Swap to nf-test * Remove spurious changes to bowtie2 * Actually revert bowtie2 * Add the rest of bowtie2 * Again * Revert bowtie2 changes * Swap to meta.id as prefix * Swap bowtie align to nftest * Update tests * ...
bowtie2-build-s_undefinedsymbol_ _ZN3tbb10interface78internal15task_arena_base19internal_initializeEv 我安装指定版本的tbb可以解决我的问题。 condainstalltbb=2020.2 参考:Conda version does not work with most recent tbb version · Issue #336 · BenLangmead/bowtie2 (github.com)...
2-4个小时吧,人类基因组。我在windows下跑了接近10个小时,后来在linux下跑,一般不超过4小时就搞定了。
bowtie2_2.3.4.1-1_amd64 NAME bowtie2-build-l - building a colorspace index for bowtie2 SYNOPSIS bowtie2-build-l[options]*<reference_in><bt2_index_base> DESCRIPTION Bowtie 2 version 2.3.4.1 by Ben Langmead (langmea@cs.jhu.edu, www.cs.jhu.edu/~langmea) reference_in comma-separate...
Hi, I have just started learning bowtie2. I encountered a problem with database indexing with bowtie2-build command: indexing runs through but the output doesn't have .rev files, thus preventing me from doing alignments to the particular...
Method/Function: build_bowtie_index 导入包: smallrnaseqaligners 每个示例代码都附有代码来源和完整的源代码,希望对您的程序开发有帮助。 示例1 def test_run(): """Run mirna test with test files""" print ('running miRNA counting test') idxpath = 'testing/indexes' if check_snap() == True:...
RNA-Seq sequence reads mapping to UCSC Human genome build 19 by TopHat v1.3.0/Bowtie v0.12.7.Li Qin ZhangDilyara CheranovaMargaret GibsonShinghua DingDaniel P. HeruthDeyu FangShui Qing Ye