Sabine P. (2013). Progress in Cell Based Assays for Botulinum Neurotoxin Detection. Curr Top Microbiol Immunol.364: 257-285.Pellett S (2013) Progress in cell based assay for botulinum neurotoxin detection. Curr Top Microbiol Immunol 364: 257-285....
Template:Drugbox Template:Infobox enzyme Botulinum toxin (BTX) is a neurotoxic protein produced by the bacterium Clostridium botulinum and related species.[1] It prevents the release of the neurotransmitter acetylcholine from axon endings at the neuromus
Botulinum Neurotoxin Serotype a Specific Cell-Based Potency Assay to Replace the Mouse Bioassay Botulinum neurotoxin serotype A (BoNT/A), a potent therapeutic used to treat various disorders, inhibits vesicular neurotransmitter exocytosis by cleaving ... E Fernández-Salas,J Wang,Y Molina,... - 《...
BOTULINUM toxinNEUROTOXICOLOGYPOLYETHYLENE glycolHYDROGELSPOLYSTYRENEBotulinum neurotoxin (BoNT) detection provides a useful model for validating cell-based neurotoxicity screening approaches, as sensitivity is dependent on functionally competent neurons and clear quantitative endpoints are available for correlating ...
The C-terminal domain of the heavy chain functions in receptor binding on the neuronal cell surface, inducing endocytotic internalization of the toxin. Once inside the endosome, protonation causes membrane insertion and chaperone/channel formation of the heavy chain coupled to light chain unfolding ...
et al. The HA proteins of botulinum toxin disrupt intestinal epithelial intercellular junctions to increase toxin absorption. Cell. Microbiol. 10, 355–364 (2008). PubMed CAS Google Scholar Sugawara, Y., Yutani, M., Amatsu, S., Matsumura, T. & Fujinaga, Y. Functional dissection of the ...
10 Each of the five toxin serotypes was propagated individually in bulk culture, followed by separation of extracellular toxin from bacterial cell mass, acid precipitation of toxin, filtration, detoxification of the active toxins using formaldehyde, and adsorption of the toxoid onto aluminum phosphate....
The potency units of DYSPORT are not interchangeable with other preparations of botulinum toxin products and, therefore, units of biological activity of DYSPORT cannot be compared to or converted into units of any other botulinum toxin products assessed with any other specific assay method [seeWARNINGS...
Selection:Studies in patients with cervical dystonia who received botulinum toxin B. Inclusion of studies was based mainly on the methods section of the trials. When available, large, well controlled trials with appropriate statistical methodology were preferred. Relevant pharmacodynamic data are also inc...
An assay for the identification of neutralizing botulinum antibodies in sera is provided which includes the steps of separating non- sodium dodecyl sulfate, non-trypsinized complex botulinum toxin in an arylamide gel by electrophoresis, the separation occurring on a basis of botulinum toxin protein si...