Define Bone-shaker. Bone-shaker synonyms, Bone-shaker pronunciation, Bone-shaker translation, English dictionary definition of Bone-shaker. n 1. an early type of bicycle having solid tyres and no springs 2. slang any decrepit or rickety vehicle Collins E
were used for osteoblasts. DAPI (Invitrogen) was applied for nuclei detection and slides were embedded in Fluorescent Mounting Media (Dako North America Inc., Carpinteria, CA, USA). Pictures were taken with an Olympus BX61 microscope (Olympus Deutschland GmbH, Hamburg, Germany) with 40-fold m...
Dimethyl sulfoxide (DMSO) (Sigma-Aldrich) was added and each plate was shaken on a plate shaker for 20 min before the absorbance was read at 570 nm using FLUOstar OPTIMA Microplate Reader (BMG Labtech, Offenburg, Germany). Multi-lineage differentiation of ASCs and BMSCs For osteogenic ...
The blocking buffer was removed from the membranes and the solution containing the conditioned media and the antibody cocktail was added and incubated on a rocking platform shaker overnight at 4 °C. On the following day, the membranes were washed with washed buffer thrice and 2 mL of ...
Bone defects resulting from trauma, disease, surgery or congenital malformations are a significant health problem worldwide. Consequently, bone is the second most transplanted tissue just after blood. Although bone grafts (BGs) have been used for decades
The CBC with a diameter of 106–212 µm was shaken with weights of 0.03 g, 0.04 g, 0.1 g, 0.2 g, 0.3 g, and 0.5 g and 50 mL of the solution in 50 mL vials in an electrical shaker with a 150 reciprocation. After 48 h of shaking, the fluoride concentration was measured in ...