Define Bone-shaker. Bone-shaker synonyms, Bone-shaker pronunciation, Bone-shaker translation, English dictionary definition of Bone-shaker. n 1. an early type of bicycle having solid tyres and no springs 2. slang any decrepit or rickety vehicle Collins E
were used for osteoblasts. DAPI (Invitrogen) was applied for nuclei detection and slides were embedded in Fluorescent Mounting Media (Dako North America Inc., Carpinteria, CA, USA). Pictures were taken with an Olympus BX61 microscope (Olympus Deutschland GmbH, Hamburg, Germany) with 40-fold m...
The blocking buffer was removed from the membranes and the solution containing the conditioned media and the antibody cocktail was added and incubated on a rocking platform shaker overnight at 4 °C. On the following day, the membranes were washed with washed buffer thrice and 2 mL of ...
Dimethyl sulfoxide (DMSO) (Sigma-Aldrich) was added and each plate was shaken on a plate shaker for 20 min before the absorbance was read at 570 nm using FLUOstar OPTIMA Microplate Reader (BMG Labtech, Offenburg, Germany). Multi-lineage differentiation of ASCs and BMSCs For osteogenic ...
Briefly, after adding 1 ml of cell suspension to the BGS, plates were agitated on an orbital shaker (Unitron, Infors HT, Basel, Switzerland) for 6 h at 180 rpm at 37 °C and in humidity conditions. Then, cells were maintained in static conditions at 37 °C in a humidified atmosphere ...
Then, the scaffolds were rinsed with distilled water in an orbital shaker for 30 min at 250 rpm to remove any undesired dust contamination before being sterilized with a pulsed light system XeMaticA-Basic-1L (Steribeam, Kehl, Germany). The latter produces pulses ranging from infrared (IR) to...
The suspension was placed in an orbital shaker (Excella E24, Eppendorf, Hamburg, Germany) at 37 °C, 70 rpm for up to 28 days. Experiments were conducted in triplicate. At each time point, samples were centrifuged, the entire volume was withdrawn and replaced with an equal amount of fresh...
Then, the scaffolds were rinsed with distilled water in an orbital shaker for 30 min at 250 rpm to remove any undesired dust contamination before being sterilized with a pulsed light system XeMaticA-Basic-1L (Steribeam, Kehl, Germany). The latter produces pulses ranging from infrared (IR) to...
The CBC with a diameter of 106–212 µm was shaken with weights of 0.03 g, 0.04 g, 0.1 g, 0.2 g, 0.3 g, and 0.5 g and 50 mL of the solution in 50 mL vials in an electrical shaker with a 150 reciprocation. After 48 h of shaking, the fluoride concentration was measured in ...
EDTA solution was changed three times a week and condyles placed on a manual shaker. Following decalcification, condyles were incubated with increasing sucrose concentrations (10–30%) and then embedded in Tissue-Tek (Sakura, Zoeterwoude, The Netherlands). Embedded condyles were cryosectioned at ...