Driver engagement is the priority rather than practicality and comfort with the GT86, though this is most definitely not a bone-shaker with little or no stowage space. Toyota's a classic; If you're looking for a great sports car experience, the GT86 could be right up your street - or ...
W. & Shaker, J. L. Lower extremity insufficiency fractures: an underappreciated manifestation of endogenous Cushing’s syndrome. Osteoporos. Int. 27, 3645–3649 (2016). CAS PubMed Google Scholar Hughes, J. M. et al. Nonsteroidal anti-inflammatory drug prescriptions are associated with ...
The blocking buffer was removed from the membranes and the solution containing the conditioned media and the antibody cocktail was added and incubated on a rocking platform shaker overnight at 4 °C. On the following day, the membranes were washed with washed buffer thrice and 2 mL of ...
were used for osteoblasts. DAPI (Invitrogen) was applied for nuclei detection and slides were embedded in Fluorescent Mounting Media (Dako North America Inc., Carpinteria, CA, USA). Pictures were taken with an Olympus BX61 microscope (Olympus Deutschland GmbH, Hamburg, Germany) with 40-fold m...
Dimethyl sulfoxide (DMSO) (Sigma-Aldrich) was added and each plate was shaken on a plate shaker for 20 min before the absorbance was read at 570 nm using FLUOstar OPTIMA Microplate Reader (BMG Labtech, Offenburg, Germany). Multi-lineage differentiation of ASCs and BMSCs For osteogenic ...
(Santa Cruz Biotechnology, Dallas, TX, USA), and the lysate was incubated for 30 min in a shaker at 4 °C. The samples were centrifuged for 15 min at 13,000 rpm at 4 °C and the supernatant was collected. The quantification with the colorimetric method (Protein Assay Dye reagent, Bio...
The suspension was placed in an orbital shaker (Excella E24, Eppendorf, Hamburg, Germany) at 37 °C, 70 rpm for up to 28 days. Experiments were conducted in triplicate. At each time point, samples were centrifuged, the entire volume was withdrawn and replaced with an equal amount of fresh...
The CBC with a diameter of 106–212 µm was shaken with weights of 0.03 g, 0.04 g, 0.1 g, 0.2 g, 0.3 g, and 0.5 g and 50 mL of the solution in 50 mL vials in an electrical shaker with a 150 reciprocation. After 48 h of shaking, the fluoride concentration was measured in ...