Kagiya (2015) noted that osteolytic bone metastasis frequently occurs in the later stages of breast, lung, and several other cancers. Osteoclasts, the only cells that resorb bone, are hijacked by tumor cells, which break down bone remodeling systems. As a result, osteolysis occurs and may ca...
Moreover, functional blockade of LPA action on its cognate receptor LPA using a pharmacological antagonist mimics the effects of silencing LPA in tumor cells in vitro and substantially reduces bone metastasis progression in animals. Overall, these results suggest that inhibition of platelet-derived LPA...
Figure 2. Enforced Expression of Jagged1 in Breast Cancer Promotes Osteolytic Bone Metastasis (A) Kaplan-Meier bone metastasis-free survival curve of mice inoculated with control or JAG1 OE SCP28 cells (n = 10). (B) Normalized BLI signals of bone metastases of mice from each experimental gr...
miR-326 associates with biochemical markers of bone turnover in lung cancer bone metastasis. Bone 2013; 52: 532–539. 135 Hassan MQ, Gordon JA, Beloti MM et al. A network connecting Runx2, SATB2, and the miR-23a,27a,24–2 cluster regulates the osteoblast differentiation program. Proc ...
In the nucleus, Notch ICD (intracellular domain) binds specific transcription factors and recruits transcriptional co-activators to induce the expression of bHLH family of genes.152Notch pathway is active in the early stages of osteoblast differentiation, also by acting on Runx2-dependent osteogenic ge...
Thiele F, Cohrs CM, Flor A, Lisse TS, Przemeck GK, Horsch M, et al. Cardiopulmonary dysfunction in the Osteogenesis imper- fecta mouse model Aga2 and human patients are caused by bone- independent mechanisms. Hum Mol Genet 2012 Aug 15;21 (16):3535-45....
Use 1 mL of cell freezing solution for each 25 cm2 or 75 cm2 flask. II. Keep the cryogenic vial at −80 °C for 3 days. III. Store the cryogenic vial in liquid nitrogen. 3.2. Peripheral Blood Mononuclear Cells Isolation Procedure Isolate peripheral blood mononuclear cells (PBMCs) from...
Anthropometric measurements were performed in the morning, with light clothing and without shoes. Body weight and height were measured by a medical scale with a stadiometer to the nearest 0.1 kg and 0.5 cm, respectively. Waist circumference was measured using a flexible, inextensible tape with an...
Conformational changes in type I collagen are associated with the relative intensities of bands at 859 cm−1, 932 cm−1, 1256 and 1279 cm−1 (amide III), and 1669 cm−1 (amide I). Proline constitutes approximately 10% of the total amino acids in collagen protein. The three parall...
by Kira J. Noordwijk 1,†, Leyi Chen 2,†, Bianca D. Ruspi 1, Sydney Schurer 1, Brittany Papa 1, Diana C. Fasanello 1, Sean P. McDonough 3, Scott E. Palmer 4, Ian R. Porter 1, Parminder S. Basran 1, Eve Donnelly