Blood or bone marrow specimens collected for this test must reach the laboratory in an expeditious manner (in our laboratory, we reject specimens that are >48 h old) and should begin RNA extraction and processing immediately. Moreover, the BCR-ABL1 quantity detected is typically given in ...
the MRI visualization of bone marrow edema patterns is useful for recreating the injury mechanism, detecting occult nondisplaced fractures, and predicting injury patterns and recovery time. Bone marrow edema of the plantar talar head is suspicious for a peritalar subluxation event and is associated wi...
DNA degradation (depolymerization) measurements of the bone marrow may be used as a supplementary test for checking the viability or restoration potency of bone-marrow cells after storage. In the freezing process, the optimum contact time between glycerol and the bone-marrow cells is 15 min. ...
Bone microvascular endothelial cells (BMECs) played an important role in the pathogenesis of glucocorticoid-induced osteonecrosis of femoral head (GCS-ONFH), and exosomes derived from bone marrow mesenchymal stem cells (BMSC-Exos) may provide an effectiv
4.1. Isolation of Primary MSCs from Bone Marrow and Collection of MSC CGMs Mouse bone marrow MSCs were isolated from the whole bone marrow aspirates of 4-week-old male Balb/c mouse (Stolbovaya, Russia). Briefly, MSCs were flushed from the bone marrow cavities of femurs and tibias with DME...
Bone marrow adipose tissue (MAT) is negatively associated with bone mass. Since osteoblasts and adipocytes are derived from the same precursor cells, adipocyte differentiation may occur at the expense of osteoblast differentiation. We used MAT-deficient KitW/W−v (MAT-) mice to determine if abse...
NS, not significant, *P<0.05, **P<0.01 and ***P<0.001, unpaired t-test. Full size image To understand the compromised peripheral B-lymphoid recovery in Mef2c-deficient mice during irradiation-induced stress, the kinetics of bone marrow recovery was assessed (Fig. 3a). At 2 days after ...
Bone marrow mesenchymal stem cells-derived exosomal miR-29b-3p regulates aging-associated insulin resistance. ACS Nano. 2019;13:2450–62. CAS PubMed Google Scholar Thomou T, Mori MA, Dreyfuss JM, Konishi M, Sakaguchi M, Wolfrum C, Rao TN, Winnay JN, Garcia-Martin R, Grinspoon SK, ...
The number of stem/progenitor cells that can be obtained for use in BTE construct preparation is often limited by the amount of donor material that can be harvested, usually from bone marrow or adipose tissue. While possible, in vitro expansion of donor cells, to reach adequate numbers for th...
We hypothesized that in knees with OA, bone marrow lesions (BMLs), synovitis, and effusion would be associated with weight-bearing and (less so with) non-weight-bearing pain independently. Methods In a cross-sectional study of persons with symptomatic knee OA using univariate and multivariate ...